Application of Bone Marrow-Derived Mesenchymal Stem Cells in a Rotator Cuff Repair Model
Application of Bone Marrow-Derived Mesenchymal Stem Cells in a Rotator Cuff Repair Model
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DOI：
10.1177/0363546509339582
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发表时间：
2009-11-01
影响因子：
4.8
通讯作者：
Rodeo, Scott A.
中科院分区：
文献类型：
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作者：
Gulotta, Lawrence V.;Kovacevic, David;Rodeo, Scott A.
背景：肩袖肌腱愈合到骨时会有瘢痕组织插入，这使得修复容易失败。本研究的目的是确定应用骨髓间充质干细胞（MSCs）是否可以改善修复后的肩袖愈合。假设：将MSCs应用于修复部位与对照组相比，在组织学和生物力学测试方面会产生上级的结果。研究设计：对照实验室研究。方法：98只刘易斯大鼠进行单侧冈上肌腱脱离和修复; 10只大鼠用于MSC收获。八只动物用于用Ad-LacZ进行细胞追踪。其余的动物接受106个MSC在纤维蛋白载体中，载体单独，或在修复部位什么都没有。在第2周和第4周处死动物进行组织学分析，以确定纤维软骨形成的量和插入处的胶原组织。生物力学testings.Results：Ad-LacZ转导的骨髓间充质干细胞治疗标本表现出更多的β-半乳糖苷酶的活性在修复部位与对照组相比，在2和4周，虽然在4周的活动是小于在2周。在任一时间点，两组之间的新软骨形成或胶原纤维组织的量没有差异。也没有差异的生物力学强度的修复，横截面积，峰值应力失败，或stiffen.Conclusion：除了骨髓间充质干细胞愈合肩袖插入部位没有改善的结构，组成，或强度的愈合肌腱附着部位，尽管有证据表明，他们是存在的，代谢活跃。
Background: Rotator cuff tendons heal to bone with interposed scar tissue, which makes repairs prone to failure. The purpose of this study was to determine if the application of bone marrow-derived mesenchymal stem cells (MSCs) can improve rotator cuff healing after repair.Hypothesis: Application of MSCs to the repair site will result in superior results compared with controls on histologic and biomechanical testing.Study Design: Controlled laboratory study.Methods: Ninety-eight Lewis rats underwent unilateral detachment and repair of the supraspinatus tendon; 10 rats were used for MSC harvest. Eight animals were used for cell tracking with Ad-LacZ. The remaining animals received either 106 MSCs in a fibrin carrier, the carrier alone, or nothing at the repair site. Animals were sacrificed at 2 and 4 weeks for histologic analysis to determine the amount of fibrocartilage formation and the collagen organization at the insertion. Biomechanical testing was also performed.Results: Specimens treated with Ad-LacZ-transduced MSCs exhibited more beta-galactosidase activity at the repair site compared with controls at both 2 and 4 weeks, although activity at 4 weeks was less than that at 2 weeks. There were no differences in the amount of new cartilage formation or collagen fiber organization between groups at either time point. There were also no differences in the biomechanical strength of the repairs, the cross-sectional area, peak stress to failure, or stiffness.Conclusion: The addition of MSCs to the healing rotator cuff insertion site did not improve the structure, composition, or strength of the healing tendon attachment site despite evidence that they are present and metabolically active.