Human immunity to Pseudomonas aeruginosa. II. Relationship between heat-stable opsonins and type-specific lipopolysaccharides.
Human immunity to Pseudomonas aeruginosa. II. Relationship between heat-stable opsonins and type-specific lipopolysaccharides.
复制标题
人体对铜绿假单胞菌的免疫力。
DOI：
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发表时间：
1972
影响因子：
6.4
通讯作者：
Lowell S. Young
中科院分区：
文献类型：
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作者：
Lowell S. Young
通过多形核白细胞吞噬和杀伤的定量试验，对Fisher、Devlin和Gnabasik的血清学分型系统描述的铜绿假单胞菌纯化的脂多糖与人血清中的热稳定性调理抗体之间的关系进行了评价。来自人类志愿者的血清显示，给予纯化的内毒素后，调理活性显著增加，同时抗体效价也随之上升。这些调理素是类型特异性的，纯化的脂多糖可以阻断每一种调理素衍生的亲本菌株的吞噬作用。血清中的调理活性可用全活菌、热灭活完整菌或脂多糖吸收来去除。这些吸收表明，在Fisher、Devlin和Gnabasik的分型系统中没有显著的交叉抗原性，但野生型铜绿假单胞菌通常含有多种内毒素抗原。铜绿假单胞菌耐热的体细胞抗原具有使生物体抵抗吞噬作用的特性；这种抵抗力可被含有特定抗体的血清中和。
The relationship between heat-stable opsonizing antibody in human serum and purified lipopolysaccharides (LPS) prepared from strains of Pseudomonas aeruginosa described in a serologic typing system by Fisher, Devlin, and Gnabasik was assessed by quantitative tests of phagocytosis and killing by polymorphonuclear leukocytes. Serum from human volunteers given purified LPS showed significantly increased opsonizing activity paralleled by rises in titers of antibody. These opsonins were type-specific, and purified LPS could block phagocytosis of the parent strain from which each was derived. The opsonizing activity in serum could be removed by absorptions with whole live bacteria, heat-killed intact bacteria, or LPS. These absorptions showed that there was no significant crossantigenicity in the typing system of Fisher, Devlin, and Gnabasik, but wild-type strains of P. aeruginosa commonly contained multiple LPS antigens. Heat-stable, somatic antigens of P. aeruginosa have properties enabling the organisms to resist phagocytosis; this resistance is neutralized by serum containing specific antibodies.