Detection of Penaeid Rod-shaped DNA Virus (PRDV) in Wild-caught Shrimp and Other Crustaceans
Detection of Penaeid Rod-shaped DNA Virus (PRDV) in Wild-caught Shrimp and Other Crustaceans
复制标题
野生虾和其他甲壳类动物中对虾杆状 DNA 病毒 (PRDV) 的检测
DOI：
10.3147/jsfp.33.373
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发表时间：
1998
期刊：
影响因子：
0.6
通讯作者：
Y. Takahashi
中科院分区：
文献类型：
--
作者：
M. Maeda;T. Itami;Atsushi Furumoto;O. Hennig;T. Imamura;M. Kondo;I. Hirono;T. Aoki;Y. Takahashi
由对虾杆状DNA病毒（PRDV）引起的对虾急性病毒血症（PAV）已成为日本对虾养殖业的主要公害。应用两步法PCR技术对野生对虾和其他甲壳类动物中的PRDV进行了检测。两步法PCR检测202尾野生虾苗中PRDV阳性51尾（25.2%）。通过注射攻击，证实了感染或携带病毒的成年虾的心脏匀浆的滤液对健康的库鲁马虾有杀死作用。11十足类甲壳类物种，这里面和外面的库鲁马虾池发生PAV捕获，PRDV阳性的两步PCR。该病毒在海岸中检出率较高。与受感染的海岸同居的库鲁马虾死亡时，甲壳内出现了白色斑点，从这些斑点中检测到了病毒。这些结果表明，PRDV可以通过水平和垂直途径传播。
Penaeid acute viremia (PAV) caused by penaeid rod-shaped DNA virus (PRDV), has been damaging kuruma shrimp (Penaeus japonicus) culture in Japan. The detection of PRDV in wild-caught shrimp and other crustaceans was conducted by using 2-step PCR. Fifty-one out of 202 wild-caught shrimp spawners showed PRDV-positive (25.2%) by 2-step PCR. The filtrate of the heart homogenate of those infected or virus-carrying adult shrimp was confirmed to kill healthy kuruma shrimp by injection challenge. Eleven decapod crustacean species, which were caught inside and outside kuruma shrimp ponds where PAV occurred, were PRDV-positive by 2-step PCR. The virus was frequently detected in the shore crabHelice tridens. The kuruma shrimp that cohabited with those infected shore crabs died showing white spots inside of the carapace, from which the virus was detected. These results suggest that PRDV can be transmitted by both horizontal and vertical routes.