Inhibition of the metallo-beta-lactamase produced from Serratia marcescens by thiol compounds
Inhibition of the metallo-beta-lactamase produced from Serratia marcescens by thiol compounds
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DOI：
10.1248/bpb.20.1136
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发表时间：
1997-11-01
影响因子：
2
通讯作者：
Arakawa, Y
中科院分区：
文献类型：
--
作者：
Goto, M;Takahashi, T;Arakawa, Y
低分子量硫醇类化合物对大肠杆菌JM 109细胞表达的粘质沙雷氏菌（Serratia marcescens）TN 9106产生的金属β-内酰胺酶（IMP-1）有很强的抑制作用。巯基乙酸和2-巯基丙酸强烈和竞争性抑制IMP-1与K，0.23和0.19 μ M，分别。2-巯基乙醇可逆地抑制IMP-1，但没有表现出简单的竞争性抑制。
Low molecular weight thiol compounds have been found to be strong inhibitors of metallo-beta-lactamase (IMP-1) produced by Serratia marcescens TN9106, which was expressed by Escherichia coli JM109 cells. Mercaptoacetic acid and 2-mercaptopropionic acid strongly and competitively inhibited IMP-1 with K, of 0.23 and 0.19 mu M, respectively. 2-Mercaptoethanol reversibly inhibited IMP-1 but did not show simple competitive inhibition.