Growth hormone treatment promotes guided bone regeneration in rat calvarial defects
Growth hormone treatment promotes guided bone regeneration in rat calvarial defects
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DOI：
10.1093/ejo/23.6.733
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发表时间：
2001-12-01
影响因子：
2.6
通讯作者：
Andreassen, TT
中科院分区：
文献类型：
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作者：
Cacciafesta, V;Dalstra, M;Andreassen, TT
本研究采用重组人生长激素（rhGH）对40只1岁雌性Wistar大鼠顶骨中部全层骨缺损（直径5 mm）进行治疗，观察了经rhGH全身治疗后，大鼠颅骨临界大小骨缺损表面覆盖聚四氟乙烯（e-PTFE）膜的生物力学强度和骨形成情况。将其随机分配到两组，每组20只动物。用颅外和颅内e-PTFE膜覆盖骨缺损。从手术当天开始，rhGH给药动物给予2.7 mg rhGH/kg/天，安慰剂注射大鼠给予等渗氯化钠。术后28天处死动物。生物力学测试通过在右侧愈合缺损中心放置直径为3.5 mm的钢冲头的冲出测试程序进行。机械测试后，去除缺损内新形成的组织，并测量干重和灰重。结果：rhGH治疗组的极限载荷、极限刚度和极限载荷时的能量吸收均显著高于对照组（P < 0.003）。rhGH治疗组的组织干重和灰分重也显著增加（P < 0.001）。新形成骨的3D重建显示，与安慰剂组相比，rhGH治疗的缺损中存在的骨体积几乎是安慰剂组的两倍。实验表明，rhGH给药增强了愈合的大鼠颅骨缺损的骨沉积和机械强度，覆盖有e-PTFE膜。
This study evaluated the biomechanical strength and bone formation in calvarial critical size bone defects covered with expanded polytetrafluoroethylene (e-PTFE) membranes in rats treated systemically with recombinant human growth hormone (rhGH).A full-thickness bone defect, 5 mm in diameter, was trephined in the central part of each parietal bone in 40 one-year-old female Wistar rats, which were randomly assigned to two groups of 20 animals each. The bone defects were covered with an exocranial and an endocranial e-PTFE membrane. From the day of operation, the rhGH-treated animals were given 2.7 mg rhGH/kg/day and the placebo-injected rats were given isotonic sodium chloride. The animals were killed 28 days after operation. The biomechanical test was performed by a punch out test procedure placing a 3.5-mm diameter steel punch in the centre of the right healed defect. After mechanical testing, the newly formed tissue inside the defect was removed and the dry and ash weights were measured. The left healed defects were used for three-dimensional (3D) reconstruction by means of micro-computer tomography (micro-CT).Ultimate load, ultimate stiffness, and energy absorption at ultimate load were significantly increased in the rhGH-treated group (P < 0.003). Also, tissue dry and ash weights were significantly augmented in the rhGH-treated group (P < 0.001). The 3D reconstruction of newly formed bone showed that there was almost twice as much bone volume present in the rhGH-treated defects compared with the placebo group. The experiment demonstrated that rhGH administration enhances bone deposition and mechanical strength of healing rat calvarial defects, covered with e-PTFE membranes.