Mouse Mandibular Retromolar Taste Buds Associated With a Mucus Salivary Gland.
Mouse Mandibular Retromolar Taste Buds Associated With a Mucus Salivary Gland.
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小鼠下颌磨牙后味蕾与粘液唾液腺相关。
DOI：
10.1093/chemse/bjab019
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发表时间：
2021
期刊：
影响因子：
3.5
通讯作者：
Tizzano,Marco
中科院分区：
文献类型：
--
作者：
Nguyen,QuanT;BeckCoburn,GraceE;Valentino,Amber;Karabucak,Bekir;Tizzano,Marco
我们的特点是最近重新发现的化学感觉结构在小鼠口腔中的下颌粘膜后部最初在20世纪80年代报道。这包括无组织的味蕾，不包含在槽内，与下面的小唾液腺的导管相关。使用整体安装准备的转基因小鼠表达绿色荧光蛋白的启动子下的味觉信号传导特异性基因，我们确定的结构包含味蕾集群和唾液腺孔在后方的每个下颌骨，远端的最后一颗磨牙和前升支。免疫组织化学分析显示，磨牙后味蕾中味觉受体Tas 2 R131和T1 R3以及味觉级联分子TrpM 5、PLCβ2和GNAT 3的表达与II型味觉细胞一致，并且GAD 1的表达与III型味觉细胞一致。此外，在包裹TrpM 5+味蕾的磨牙后粘膜组织中观察到神经元标记物--降钙素基因相关肽。RT-PCR结果显示磨牙后味蕾表达所有3个tas 1 r基因和35个tas 2 r基因中的28个，以及味觉信号转导基因gnat 3、plcb 2和trpm 5，使磨牙后味蕾与其他舌、腭味蕾相似。最后，组织化学证实下颌磨牙后分泌腺为粘液型小唾液腺。因此，下颌磨牙后味觉结构可能在味觉中发挥作用，并代表味觉障碍的潜在新药理学靶点。
We have characterized a recently rediscovered chemosensory structure at the rear of the mandibular mucosa in the mouse oral cavity originally reported in the 1980s. This consists of unorganized taste buds, not contained within troughs, associated with the ducts of an underlying minor salivary gland. Using whole-mount preparations of transgenic mice expressing green fluorescent protein under the promoter of taste-signaling-specific genes, we determined that the structure contains taste bud clusters and salivary gland orifices at the rear of each mandible, distal to the last molar and anterior to the ascending ramus. Immunohistochemical analysis shows in the retromolar taste buds expression of the taste receptors Tas2R131 and T1R3 and taste cascade molecules TrpM5, PLCβ2, and GNAT3, consistent with type II taste cells, and expression of GAD1, consistent with type III taste cells. Furthermore, the neuronal marker, calcitonin gene-related peptide, in retromolar mucosa tissue wrapping around TrpM5+ taste buds was observed. RT–PCR showed that retromolar taste buds express all 3 mousetas1rgenes, 28 of the 35tas2rgenes, and taste transduction signaling genesgnat3, plcb2, andtrpm5, making the retromolar taste buds similar to other lingual and palate taste buds. Finally, histochemistry demonstrated that the mandibular retromolar secretory gland is a minor salivary gland of mucous type. The mandibular retromolar taste structure may thus play a role in taste sensation and represent a potential novel pharmacological target for taste disorders.