Multiple Resistance to Herbicides from Four Site-of-Action Groups in Waterhemp (Amaranthus tuberculatus)
Multiple Resistance to Herbicides from Four Site-of-Action Groups in Waterhemp (Amaranthus tuberculatus)
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DOI：
10.1614/ws-d-12-00166.1
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发表时间：
2013-07-01
期刊：
影响因子：
2.5
通讯作者：
Tranel, Patrick J.
中科院分区：
文献类型：
--
作者：
Bell, Michael S.;Hager, Aaron G.;Tranel, Patrick J.
2006年和2007年，伊利诺斯州两个县的农民报告说，他们未能用草甘膦控制水大麻。随后的现场试验表明，这些种群可能对多种除草剂具有抗性。因此，进行了温室试验，以确认其对草甘膦的抗性，并测试其对其他除草剂的多重抗性，包括阿特拉津、氟虫胺、乳虫胺和伊玛唑莫。在草甘膦剂量反应实验中，两个种群对先前表征的草甘膦抗性种群（MO1）的反应相似。这两个伊利诺斯州的人群也表现出对乙酰乳酸合酶（ALS）抑制剂imazamox的高频率耐药性。此外，其中一个种群对阿特拉津和原卟啉原氧化酶（PPO）抑制剂乳芬均表现出高频率的耐药性。此外，通过顺序施用和罐式混合施用除草剂的组合，从一个种群中鉴定出对所有四个作用位点群的除草剂具有抗性的单个植物。分子实验提供了抗性机制的初步表征，并证实了两个种群中存在多种抗性性状。这两个种群都含有W574L ALS突变和Delta G210 PPO突变，先前分别显示对ALS和PPO抑制剂具有耐药性。在这两个人群中，阿特拉津耐药性被怀疑是基于代谢的，因为没有发现三嗪靶位点突变。在一个人群中发现了先前报道的赋予草甘膦抗性的P106S EPSPS突变。该突变在抗性植物和敏感植物中均有发现；然而，因此需要更多的研究来确定草甘膦抗性机制。这是美国已知的第一个杂草种群对四种作用位点的除草剂具有多重抗性的案例。
In 2006 and 2007, farmers from two counties in Illinois reported failure to control waterhemp with glyphosate. Subsequent onsite field experiments revealed that the populations might be resistant to multiple herbicides. Greenhouse experiments therefore were conducted to confirm glyphosate resistance, and to test for multiple resistance to other herbicides, including atrazine, acifluorfen, lactofen, and imazamox. In glyphosate dose-response experiments, both populations responded similarly to a previously characterized glyphosate-resistant population (MO1). Both Illinois populations also demonstrated high frequencies of resistance to the acetolactate synthase (ALS) inhibitor, imazamox. Additionally, one of the populations demonstrated high frequencies of resistance to both atrazine and the protoporphyrinogen oxidase (PPO) inhibitor, lactofen. Furthermore, using combinations of sequential and tank-mix herbicide applications, individual plants resistant to herbicides spanning all four site-of-action groups were identified from one population. Molecular experiments were performed to provide an initial characterization of the resistance mechanisms and to provide confirmation of the presence of multiple resistance traits within the two populations. Both populations contained the W574L ALS mutation and the Delta G210 PPO mutation, previously shown to confer resistance to ALS and PPO inhibitors, respectively. Atrazine resistance in both populations is suspected to be metabolism-based, because a triazine target-site mutation was not identified. A P106S EPSPS mutation, previously reported to confer glyphosate resistance, was identified in one population. This mutation was identified in both resistant and sensitive plants from the population; however, and so more research is needed to determine the glyphosate-resistance mechanism(s). This is the first known case of a weed population in the United States possessing multiple resistance to herbicides from four site-of-action groups.