Discrepancies in Epstein-Barr virus association at presentation and relapse of classical Hodgkin's disease: Impact on pathogenesis
Discrepancies in Epstein-Barr virus association at presentation and relapse of classical Hodgkin's disease: Impact on pathogenesis
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DOI：
10.1023/a:1008363805242
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发表时间：
2000-04-01
影响因子：
50.5
通讯作者：
Naresh, KN
中科院分区：
文献类型：
--
作者：
Nerurkar, AY;Vijayan, P;Naresh, KN
背景：虽然EB病毒（EBV）与经典霍奇金病（cHD）有关，但只有一部分cHD病例显示EBV相关。虽然有证据表明EBV在cHD演变的早期阶段起着至关重要的作用，但我们不清楚EBV的持续存在是否对肿瘤的维持至关重要。我们已经解决了这个问题，通过调查EBV协会在配对样本中获得的cHD在presentation和relations.Materials和方法：我们分析了23例cHD在presentation和复发的淋巴结活检样本。石蜡切片进行EBER-1探针原位杂交和抗潜伏膜蛋白-1（LMP-1）抗体免疫染色。结果：14例初发和复发病例中均发现EB病毒相关性。1例复发时EBV相关性丧失。PCR可分析的DNA从四个EBV相关病例的表现和复发样本中获得。EBNA 3C扩增产物与EB病毒A型毒株一致。结论：1例EB病毒感染者的临床表现与复发之间无关联，提示EB病毒诱导的淋巴瘤发生存在“打了就跑”的机制。虽然EBV可能是必不可少的早期演变的cHD，它可能不是必需的维持肿瘤克隆。我们的研究还提出了一个推测，即一定比例的EBV阴性cHD可能代表临床表现前肿瘤中EBV的丢失。另外，一种未鉴定的嗜淋巴细胞病毒而不是EBV可能在EBV阴性cHD中发挥致病作用。
Background: Though Epstein-Barr virus (EBV) has been linked to classical Hodgkin's disease (cHD), only a proportion of cHD cases show EBV-association. While there has been evidence to suggest a crucial role for EBV in the early phase of cHD evolution, we are unclear whether continued presence of EBV is essential for the maintenance of the neoplasm. We have addressed this issue by investigating the EBV-association in paired samples of cHD obtained at presentation and relapse.Materials and methods: We have analysed lymph node biopsy samples from 23 cases of cHD at presentation and relapse. In situhybridization with EBER-1 probe and immunostaining with anti latent membrane protein-1 (LMP-1) antibody was performed on the paraffin sections. PCR for EBNA 3C gene was performed for EBV subtype analysis on the DNA extracted from paraffin sections.Results: EBV-association was documented in both the presentation and relapse samples of 14 cases. One case showed loss of EBV-association at relapse. PCR analysable DNA was obtained from both presentation and relapse samples in four of the EBV-associated cases. The EBNA 3C amplimer corresponded to type A strain of EBV in all the samples.Conclusion: Loss of EBV-association between presentation and relapse seen in one case implies a hit and run mechanism in EBV-induced lymphomagenesis. Though EBV may be essential early in the evolution of cHD, it may not be required for maintenance of the neoplastic clone. Our study also brings out a speculation that a proportion of EBV-negative cHD could represent loss of EBV in the tumour prior to clinical presentation. Alternatively, an unidentified lymphotropic virus other than EBV might play a pathogenetic role in EBV-negative cHD.