Ontogeny and regulation of rat placental estrogen receptor.
Ontogeny and regulation of rat placental estrogen receptor.
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大鼠胎盘雌激素受体的个体发育和调节。
DOI：
10.1210/endo-102-1-273
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发表时间：
1978
期刊：
影响因子：
4.8
通讯作者：
S. Glasser
中科院分区：
文献类型：
--
作者：
S. McCormack;S. Glasser
交换条件下的大鼠胎盘匀浆的细胞质和核组分的Scatchard分析表明，雌激素受体（RE）是目前在一个有限的时间内在胎盘的基底区妊娠中期处于高水平。胎盘RE的消失似乎受到严格的调节。从妊娠第9-15天（第0天=精子阳性日），胞质RE从30，000个位点/细胞下降到600个位点/细胞，核RE从3400个位点/细胞下降到200个位点/细胞。我们试图通过控制激素水平和/或增加胎盘基底层滋养层巨细胞的比例来改变第12-15天的RE水平。可实现对RE水平的两个影响。首先，在卵巢切除术降低雌激素的实验条件下，胞浆RE的消失延迟了2天。胞液RE水平暂时升高可能是由于雌激素不足，不能将RE转运到细胞核内，而不是RE合成增加，因为RE下降到低水平时，细胞内RE水平可能升高。
Scatchard analysis of cytosol and nuclear fractions of rat placental homogenates under exchange conditions showed that an estrogen receptor (RE) was present at high levels during a limited period of midpregnancy in the basal zone of the placenta. The disappearance of placental RE appeared to be under strict regulation. From days 9–15 of pregnancy (day 0 = sperm-positive day) cytosol RE fell from 30,000 to 600 sites/cell and nuclear RE from 3400 to 200 sites/cell. We attempted to change RE levels from days 12–15 by manipulating hormone levels and/or increasing the proportion of trophoblast giant cells in the basal zone of the placenta. Two effects on RE levels could be achieved. First, the disappearance of cytosol RE was delayed by 2 days under experimental conditions in which estrogen was lowered by ovariectomy. The temporarily increased levels of cytosol RE may have been due to insufficient estrogen to translocate RE into the nucleus rather than to increased synthesis of RE, inasmuch as RE fell to low le...