A whole-genome radiation hybrid map of the dog genome
A whole-genome radiation hybrid map of the dog genome
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DOI：
10.1006/geno.1998.5602
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发表时间：
1998-12-15
期刊：
影响因子：
4.4
通讯作者：
Galibert, F
中科院分区：
文献类型：
--
作者：
Priat, C;Hitte, C;Galibert, F
通过对126个辐射杂种细胞系的400个标记（包括218个基因和182个微卫星）进行分型，构建了犬全基因组辐射杂种（RH）图谱。已经确定了57个RH组的lod评分大于6，并以大于1000：1的优势订购了180个框架标志。相邻标记之间的平均间距为23 cR（5000），估计物理距离为3.8 Mb。14组已被分配到9条犬染色体，和RH和遗传组的比较允许成功的桥接两种类型的数据在一个地图上组成的31 RH和13同线RH组。比较犬、人、小鼠和猪的基因图谱，发现保守的同线性区域。这个综合地图，覆盖了估计80%的狗的基因组，应该证明是一个强大的工具，定位和识别基因牵连的病理和表型性状。(C)北京：科学出版社.
A whole genome radiation hybrid (RH) map of the canine genome was constructed by typing 400 markers, including 218 genes and 182 microsatellites, on a panel of 126 radiation hybrid cell lines. Fifty-seven RH groups have been determined with lod scores greater than 6, and 180 framework landmarks were ordered with odds greater than 1000:1. Average spacing between adjacent markers is 23 cR(5000), an estimated physical distance of 3.8 Mb. Fourteen groups have been assigned to 9 of the canine chromosomes, and a comparison of RH and genetic groups allowed the successful bridging of both types of data on one map composed of 31 RH and 13 syntenic RH groups. Comparison of canine, human, mouse, and pig maps underlined regions of conserved synteny. This integrated map, covering an estimated 80% of the dog genome, should prove a powerful tool for localizing and identifiying genes implicated in pathological and phenotypical traits. (C) 1998 Academic Press.