Identification of potential novel hosts and the risk of infection with lymphocytic choriomeningitis virus in humans in Gabon, Central Africa
Identification of potential novel hosts and the risk of infection with lymphocytic choriomeningitis virus in humans in Gabon, Central Africa
复制标题
中非加蓬人类潜在新宿主的鉴定和感染淋巴细胞性脉络膜脑膜炎病毒的风险
DOI：
10.1016/j.ijid.2021.02.105
复制
发表时间：
2021
影响因子：
8.4
通讯作者：
Le
中科院分区：
文献类型：
--
作者：
Ushijima Y;Abe H;Ozeki T;Ondo GN;Mbadinga MJVM;Bikangui R;Nze-Nkogue C;Akomo-Okoue EF;Ella GWE;Koumba LBM;Nso BCBB;Mintsa-Nguema R;Makouloutou-Nzassi P;Makanga BK;Nguelet FLM;Zadeh VR;Urata S;Mbouna AVN;Massinga-Loembe M;Agnandji ST;Le
目的 淋巴细胞脉络膜脑膜炎病毒（LCMV）是一种人类致病性沙粒病毒，在世界范围内分布。然而，非洲尚未报告人间病例。本研究旨在调查中非加蓬的LCMV感染现状及潜在风险。方法筛选492份人体样本，分别采用逆转录定量PCR和酶联免疫吸附试验（ELISA）检测LCMV基因组RNA和抗LCMV IgG抗体。使用中和测定进一步检查 ELISA 阳性样品。还对 326 份动物样本（包括啮齿类动物、鼩鼱和丛林肉）中的病毒 RNA 和抗体进行了分析。结果虽然在人类样本中未检测到 LCMV RNA，但总体血清阳性率为 21.5%，并且在男性和成年人群中显着较高。中和测定鉴定出七个具有中和活性的样品。在一种啮齿动物（Lophuromys sikapusi）和一种豪猪中检测到 LCMV RNA，在四种啮齿动物和三种鼩鼱中检测到抗 LCMV IgG 抗体。结论这项研究首次确定了加蓬 LCMV 的血清流行率，并揭示了半城市周边地区的当地啮齿动物、鼩鼱和豪猪存在感染风险。因此，LCMV 感染应被视为非洲的一个重大公共卫生问题。
ObjectivesLymphocytic choriomeningitis virus (LCMV), a human pathogenic arenavirus, is distributed worldwide. However, no human cases have been reported in Africa. This study aimed to investigate the current situation and potential risks of LCMV infection in Gabon, Central Africa.MethodsA total of 492 human samples were screened to detect LCMV genome RNA and anti-LCMV IgG antibodies using reverse transcription-quantitative PCR and enzyme-linked immunosorbent assay (ELISA), respectively. ELISA-positive samples were further examined using a neutralization assay. Viral RNAs and antibodies were also analyzed in 326 animal samples, including rodents, shrews, and bushmeat.ResultsWhile no LCMV RNA was detected in human samples, the overall seroprevalence was 21.5% and was significantly higher in male and adult populations. The neutralization assay identified seven samples with neutralizing activity. LCMV RNA was detected in one species of rodent (Lophuromys sikapusi) and a porcupine, and anti-LCMV IgG antibodies were detected in four rodents and three shrews.ConclusionsThis study determined for the first time the seroprevalence of LCMV in Gabon, and revealed that local rodents, shrews, and porcupines in areas surrounding semi-urban cities posed an infection risk. Hence, LCMV infection should be considered a significant public health concern in Africa.