ORDERED RESTRICTION MAPS OF SACCHAROMYCES-CEREVISIAE CHROMOSOMES CONSTRUCTED BY OPTICAL MAPPING
ORDERED RESTRICTION MAPS OF SACCHAROMYCES-CEREVISIAE CHROMOSOMES CONSTRUCTED BY OPTICAL MAPPING
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DOI：
10.1126/science.8211116
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发表时间：
1993-10-01
期刊：
影响因子：
56.9
通讯作者：
WANG, YK
中科院分区：
文献类型：
--
作者：
SCHWARTZ, DC;LI, XJ;WANG, YK
一种基于光学显微镜的技术，用于快速构建有序的染色体物理图谱已经开发出来。在琼脂糖凝胶中固定后，通过荧光显微镜对伸长的单个DNA分子（大小约为0.2至1.0兆碱基）的限制性酶消化进行成像。通过相对荧光强度和表观分子轮廓长度确定所得单个限制性片段的大小。然后从基因组DNA创建有序限制性图谱，而不依赖于用于杂交或分析凝胶电泳的克隆或扩增序列。初步应用光学定位酿酒酵母染色体。
A light microscope-based technique for rapidly constructing ordered physical maps of chromosomes has been developed. Restriction enzyme digestion of elongated individual DNA molecules (about 0.2 to 1.0 megabases in size) was imaged by fluorescence microscopy after fixation in agarose gel. The size of the resulting individual restriction fragments was determined by relative fluorescence intensity and apparent molecular contour length. Ordered restriction maps were then created from genomic DNA without reliance on cloned or amplified sequences for hybridization or analytical gel electrophoresis. Initial application of optical mapping is described for Saccharomyces cerevisiae chromosomes.