FRET-based carbazole-fluorescein ionic nanoparticle for use as an effective bioimaging agent
FRET-based carbazole-fluorescein ionic nanoparticle for use as an effective bioimaging agent
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DOI：
10.55092/bm20230006
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发表时间：
2023-07
期刊：
影响因子：
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通讯作者：
Amanda Jalihal;Hannah Krehbiel;Samantha Macchi;Mavis Forson;Mujeebat Bashiru;Thuy Le;Caroline Kornelsen;Noureen Siraj
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文献类型：
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作者：
Amanda Jalihal;Hannah Krehbiel;Samantha Macchi;Mavis Forson;Mujeebat Bashiru;Thuy Le;Caroline Kornelsen;Noureen Siraj
福斯特共振能量转移（FRET）为基础的系统是广泛适用于许多领域的利益。在这项研究中，一种新的FRET基离子材料（IM）通过配对咔唑咪唑阳离子（CI+）与荧光素阴离子（FL 2-）通过一个简单的离子交换方法。将所得IM（[Cl]2[Fl]）在水性介质中转化为离子纳米颗粒（INP），用于生物成像应用的实际用途。详细研究了母体染料[Cl]2[Fl]和INP的物理化学性质。在合成的材料中计算所有FRET参数。[Cl]2[Fl]表现出显著的光谱重叠积分和理想的理论FRET距离。FRET机制的存在通过观察到的供体荧光寿命的降低和[Cl]2[Fl]中的中等FRET效率来验证。评价由[Cl]2[Fl]形成的INP用作荧光pH探针和生物成像剂。在一系列pH研究中计算INP的FRET效率，其表明在生理pH下效率最高。而在高酸性和碱性条件下没有观察到FRET现象。[Cl]2[Fl]的pH依赖性生物物理性质被监测，并允许作为用于检测生物系统中的酸性组织的荧光探针的潜在应用。与母体染料相比，具有FRET能力的INP在体外显示出上级的生物成像能力。
Fӧrster resonance energy transfer (FRET)-based systems are widely applicable in many areas of interest. In this study, a novel FRET-based ionic material (IM) was synthesized by pairing carbazole imidazolium cation (CI+) with fluorescein anion (Fl2−) through a simple ion-exchange method. The resulting IM ([CI]2[Fl]) was converted into an ionic nanoparticle (INP) in aqueous media for practical use for bioimaging application. The photophysical properties of the parent dyes, [CI]2[Fl], and INP were studied in detail. All FRET parameters were calculated in the synthesized material. [CI]2[Fl] exhibited a significant spectral overlap integral and an ideal theoretical FRET distance. The presence of the FRET mechanism was verified by the observed decrease in donor fluorescence lifetime and a moderate FRET efficiency in [CI]2[Fl]. The INP formed from [CI]2[Fl] was evaluated for use as a fluorescent pH probe and bioimaging agent. FRET efficiency of INP is calculated in a series of pH studies which indicates the highest efficiency at physiological pH. Whereas no FRET phenomenon is observed in highly acidic and basic conditions. The pH-dependent photophysical properties of [CI]2[Fl] are monitored and allow for the potential application as a fluorescent probe for the detection of acidic tissues in biological systems. The FRET-capable INP showed superior bioimaging capability in vitro as compared to the parent dye.