Relationship between fertilization rate and early apoptosis in sperm population of infertile individuals
Relationship between fertilization rate and early apoptosis in sperm population of infertile individuals
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DOI：
10.1111/and.12038
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发表时间：
2014-02-01
期刊：
影响因子：
2.4
通讯作者：
Nasr-Esfahani, M. H.
中科院分区：
文献类型：
--
作者：
Tavalaee, M.;Deemeh, M. R.;Nasr-Esfahani, M. H.
精子膜的完整性对受精至关重要，其中磷脂酰丝氨酸（PS）起着核心作用。PS的外化发生在获能和顶体反应过程中。从内膜到外膜的EPS被认为是早期细胞凋亡的标志。因此，EPS在精子中可能具有双重功能。本研究评估了选择ICSI的夫妇中EPS与受精、胚胎质量和妊娠结局之间的关系。从43名ICSI候选人中收集精液样本，并根据世界卫生组织精液参数指南进行评估。膜联蛋白V和碘化丙啶（PI）染色检测EPS。用色霉素A3 （CMA3）染色评价鱼精蛋白缺乏症。受精率与膜联蛋白阳性PI-阴性(An（+)PI(-)）精子呈显著正相关。缺乏蛋白蛋白的精子百分比与受精率呈显著负相关。此外，受精率高于和低于50%个体的An(+)PI（-）精子百分比差异显著。孕妇伴侣精液中膜联蛋白阳性PI-阳性(An（+)PI(+)）精子百分比与非孕妇伴侣有显著差异。综上所述，如果An(+)PI（-）是获能化的标志，而An(+)PI（+）是细胞凋亡的标志，结果表明，接受获能化能力越强的精液样本在icsi后成功受精的机会越大。在获能前的授精样本中存在高比例的凋亡精子可能会降低怀孕的机会。
Integrity of the sperm membrane, of which phosphatidyl serine (PS) plays a central role, is essential for fertilization. The externalisation of PS (EPS) occurs during capacitation and the acrosome reaction. EPS, from the inner to the outer membrane, is considered as a sign of early apoptosis. Therefore, EPS may have a dual function in sperm. This study has evaluated the relationship between EPS and fertilization, embryo quality and pregnancy outcomes in couples who were candidates for ICSI. Semen samples were collected from 43 ICSI candidates and assessed according to World Health Organization guidelines for semen parameters. EPS was assessed by Annexin V and propidium iodide (PI) staining. Protamine deficiency was assessed by chromomycin A3 (CMA3) staining. A significant positive correlation was observed between the percentages of fertilization and annexin-positive PI-negative (An(+)PI(-)) sperm. There was a significant negative correlation between the percentages of protamine-deficient sperm with the percentage of fertilization. In addition, the percentage of An(+)PI(-) sperm in individuals with fertilization rates higher and lower than 50% significantly differed. The percentage of annexin-positive PI-positive (An(+)PI(+)) sperm in semen of the partners of pregnant women significantly differed from the partners of nonpregnant women. In conclusion, if An(+)PI(-) is a sign of capacitation and An(+)PI(+) is a sign of apoptosis, the results suggest that semen samples with a higher ability to undergo capacitation have a higher chance to result in successful fertilization post-ICSI. The presence of a high percentage of apoptotic sperm in the insemination sample before capacitation may reduce the chances of pregnancy.