Gravity-dependent differentiation and root coils in Arabidopsis thaliana wild type and phospholipase-A-I knockdown mutant grown on the International Space Station
Gravity-dependent differentiation and root coils in Arabidopsis thaliana wild type and phospholipase-A-I knockdown mutant grown on the International Space Station
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DOI：
10.1111/plb.12123
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发表时间：
2014-01-01
期刊：
影响因子：
3.9
通讯作者：
Pietrzyk, P.
中科院分区：
文献类型：
--
作者：
Scherer, G. F. E.;Pietrzyk, P.
拟南芥根在45度倾斜的琼脂中以1-g生长在波浪状图形中。除了波，根卷曲的形成观察到在几个突变体中妥协的向地性和/或生长素运输。patatin相关磷脂酶-A-I的敲低突变体ppla-I-1在根向地性方面延迟并且形成更多的根卷曲。三个已知的因素有助于波动：回旋，重力感应和负向触性。在微重力条件下，野生型（WT）和突变型根的重力感应和触变性和回旋运动（已知在微重力条件下会减慢，并可能导致WT和突变型中的波减少或卷曲增加）被剥夺。为了解决这个问题，突变体ppla-I-1和WT在国际空间站的BIOLAB设施中生长。在1-g中，两种类型的根仅表现出波浪状。在微重力下的第一个实验中，突变体在9天后形成的卷曲比在1-g中形成的卷曲多得多，但WT也形成了几个卷曲。在微重力环境下24天后，两种类型的螺旋都很多，突变体的螺旋略多。在第二个实验中，在微重力下9天后，只有突变体形成了卷曲，WT长出了弯曲的根。微重力条件下，突变体根表面的细胞纵列旋转（CFR）比WT降低，因此对卷曲不重要。几个额外的发育反应（下胚轴伸长，侧根形成，子叶扩展）被认为是重力的影响。我们试探性地讨论这些在生长素运输，这是众所周知的，通过缺乏重力的干扰的背景下。
Arabidopsis roots on 45 degrees tilted agar in 1-g grow in wave-like figures. In addition to waves, formation of root coils is observed in several mutants compromised in gravitropism and/or auxin transport. The knockdown mutant ppla-I-1 of patatin-related phospholipase-A-I is delayed in root gravitropism and forms increased numbers of root coils. Three known factors contribute to waving: circumnutation, gravisensing and negative thigmotropism. In microgravity, deprivation of wild type (WT) and mutant roots of gravisensing and thigmotropism and circumnutation (known to slow down in microgravity, and could potentially lead to fewer waves or increased coiling in both WT and mutant). To resolve this, mutant ppla-I-1 and WT were grown in the BIOLAB facility in the International Space Station. In 1-g, roots of both types only showed waving. In the first experiment in microgravity, the mutant after 9days formed far more coils than in 1-g but the WT also formed several coils. After 24days in microgravity, in both types the coils were numerous with slightly more in the mutant. In the second experiment, after 9days in microgravity only the mutant formed coils and the WT grew arcuated roots. Cell file rotation (CFR) on the mutant root surface in microgravity decreased in comparison to WT, and thus was not important for coiling. Several additional developmental responses (hypocotyl elongation, lateral root formation, cotyledon expansion) were found to be gravity-influenced. We tentatively discuss these in the context of disturbances in auxin transport, which are known to decrease through lack of gravity.