IMPAIRMENT OF CELL-MEDIATED-IMMUNITY FUNCTIONS BY DIETARY ZINC-DEFICIENCY IN MICE
IMPAIRMENT OF CELL-MEDIATED-IMMUNITY FUNCTIONS BY DIETARY ZINC-DEFICIENCY IN MICE
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DOI：
10.1073/pnas.76.1.457
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
GOOD, RA
中科院分区：
文献类型：
--
作者：
FERNANDES, G;NAIR, M;GOOD, RA
分析了缺锌饮食[锌(-)]小鼠、成对饲喂含锌[锌(+)]饮食的小鼠、随意饲喂锌(+)饮食的小鼠和临时喂食实验室食物的小鼠的几个免疫学特征。6~8wk龄A/Jax、C57BL/Ks和CBA/H小鼠在饲喂锌(-)饲料后4~8wk内体重减轻，淋巴组织重量降低，胸腺明显退缩。大约50%的锌(-)饮食的小鼠出现了严重的肢端皮炎、肠病(尾巴和爪子上的皮损)和腹泻。用锌(+)饮食配对喂养的小鼠没有表现出任何这些症状。锌(-)饮食的小鼠表现出以下免疫缺陷：体内免疫后对绵羊红细胞的空斑形成细胞抑制，体内免疫后T[胸腺来源]杀伤细胞对EL-4[小鼠淋巴瘤]肿瘤细胞的抑制作用，以及自然杀伤细胞活性低下。在锌(-)饮食的小鼠中，抗体依赖的细胞介导的对鸡红细胞的细胞毒性是正常的。用EL-4同种异体淋巴瘤细胞进行体外免疫，未发现T杀伤细胞活性低下。在锌(-)动物的脾和淋巴结中，Thy1.2+细胞的相对和绝对数量进行性减少，而Fc受体的细胞比例相对增加。锌显然是维持体内正常T细胞和其他免疫功能所必需的元素。
Several immunologic features were analyzed in mice on a Zn deficient diet [Zn(-)], in mice pair-fed a diet containing Zn [Zn(+)], in mice fed a Zn(+) diet ad lib and in mice fed laboratory chow ad lib. When placed on a Zn(-) diet, 6-8 wk old A/Jax, C57BL/Ks and CBA/H mice showed loss of body weight, low lymphoid tissue weight and profound involution of the thymus within 4-8 wk after initiation of the regimen. Approximately 50% of the mice on the Zn(-) diet developed severe acrodermatitis enteropathica (lesions on tail and paws) and diarrhea. Pair-fed mice on the Zn(+) diet did not show any of these symptoms. Mice on the Zn(-) diet showed the following immune deficiencies: depressed plaque-forming cells against sheep erythrocytes after in vivo immunization, depressed T [thymus-derived] killer cell activity against EL-4 [mouse lymphoma] tumor cells after in vivo immunization and low natural killer cell activity. Antibody-dependent cell-mediated cytotoxicity against chicken erythrocytes was normal in the mice on the Zn(-) diet. Deficiency of T killer cell activity was not observed when immunization with El-4 allogeneic lymphoma cells was carried out in vitro. Progressive loss of relative and absolute number of Thy 1.2 + cells and a proportionate relative increase in cells bearing Fc receptors was seen in spleen and lymph nodes of Zn(-) animals. Zn is apparently an essential element for maintenance of normal T cell and other immune function in vivo.