Nonoptically probing near-field microscopy for the observation of biological living specimens
Nonoptically probing near-field microscopy for the observation of biological living specimens
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用于观察生物活体标本的非光学探测近场显微镜
DOI：
10.1063/1.1350965
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发表时间：
2001
影响因子：
4
通讯作者：
O. Nakamura
中科院分区：
文献类型：
--
作者：
Y. Kawata;M. Murakami;C. Egami;O. Sugihara;N. Okamoto;M. Tsuchimori;O. Watanabe;O. Nakamura
我们利用我们最近开发的非光学探测近场显微镜以亚波长分辨率观察活体标本。在近场显微镜中，样品附近的光场分布被记录为感光薄膜的表面形貌，并用原子力显微镜读出形貌分布。由于近场显微镜不需要扫描探针尖端来照明或检测或散射光，因此可以观察移动的生物样本和快速现象。我们演示了以亚波长分辨率观察移动的草履虫和眼虫。还证明了对眼虫细胞内细胞核的观察。
We present the observation of living specimens with subwavelength resolution by using the nonoptically probing near-field microscopy we have developed recently. In the near-field microscope, the optical field distributions near the specimens are recorded as the surface topography of a photosensitive film, and the topographical distributions are readout with an atomic-force microscopy. Since the near-field microscope does not require the scanning of a probe tip for illumination or detection or scattering of light, it is possible to observe moving biological specimens and fast phenomena. We demonstrate the observation of a moving paramecium and euglena gracilis with subwavelength resolution. The observation of the nucleus inside a euglena cell was also demonstrated.