Repeated-batch fermentative for bio-hydrogen production from.

Repeated-batch fermentative for bio-hydrogen production from.
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重复批次发酵用于生物制氢。

DOI:
10.3923/pjbs.2007.1782.1789
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发表时间:
2007
期刊:
Pakistan journal of biological sciences : PJBS
影响因子:
--
通讯作者:
Samart Moonamart
Samart Moonamart
中科院分区:
--
文献类型:
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作者:
S. Sangyoka;A. Reungsang;Samart Moonamart

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在10 L反应器中,采用间歇发酵和重复分批发酵,在室温、pH 6.0、工作体积为8 L的条件下,利用热处理的UASB颗粒污泥对木薯废水进行厌氧制氢。在间歇反应器中获得了比产氢潜力、产氢量和最大产氢速率,分别为39、304.81、0.22mLmg-Coid(-1)和851.84 mLh-1。当发酵液中的葡萄糖浓度耗尽到150-250 mg L(-1),相当于初始葡萄糖浓度的10-15%时,进行重复分批。重复间歇反应器以3种不同的进料/出料速率运行,即25%、50%和75%。结果表明,木薯废水制氢的适宜进料/出料比为75%。最高的产氢量、最大潜在产氢量、相对较高的最大产氢速率、较高的最大比产氢速率和较短的滞后时间分别为0.0094、12、532.80、540.46、3.5mLg-VSS(-1)和5.31h。主要的可溶性代谢物是醋酸和丁酸。结果表明,重复分批发酵比分批发酵更能有效地利用木薯废水产氢。
Anaerobic hydrogen production from cassava wastewater by heat-treated UASB granules was conducted in a 10 L bioreactor with a working volume of 8 L at room temperature and pH 6.0 by batch and repeated-batch fermentations. Specific hydrogen production potential, hydrogen yield and the maximum hydrogen production rate of 39, 304.81 mL, 0.22 mL mg-COID(-1) and 851.84 mL h(-1), respectively, were obtained in a batch reactor. A repeated-batch was conducted when the glucose concentration in fermentative broth was depleted to 150-250 mg L(-1) which equivalent to 10-15% of initial glucose concentration. Repeated-batch reactor was operated at 3 different feed-in/feed-out rates i.e., 25, 50 and 75%. Results revealed that a suitable feed-in/feed out rate for production of hydrogen from cassava wastewater was at 75%. This was indicated by the highest hydrogen yield, the highest potential maximal amount of hydrogen produced, a relatively high maximum hydrogen production rate, a relatively high maximum specific hydrogen production rate and a relatively short lag time of 0.0094 mL mg-COD(-1), 12,532.80 mL, 540.46 mL h(-1), 3.5 mL g-VSS(-1) h and 5.31 h, respectively. Major soluble metabolites were acetic and butyric acids. Our results indicated that repeated batch fermentation was more effective in producing hydrogen from cassava wastewater than batch fermentation.