Galβ1-6Gal, antigenic epitope which accounts for serological cross-reaction in diagnosis of Echinococcus multilocularis infection
Galβ1-6Gal, antigenic epitope which accounts for serological cross-reaction in diagnosis of Echinococcus multilocularis infection
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DOI：
10.1111/j.1365-3024.2009.01129.x
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发表时间：
2009-08-01
影响因子：
2.2
通讯作者：
Takeda, T.
中科院分区：
文献类型：
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作者：
Yamano, K.;Goto, A.;Takeda, T.
寄生蠕虫表达多种抗原性碳水化合物，这常常是血清学交叉反应的原因。在血清学诊断中，必须检查目标寄生虫与其他寄生虫之间的交叉反应性，以评估诊断性能。我们的前期研究表明，Gal β 1- 6 Gal序列是多房棘球绦虫（Echinococcus multilocularis，Em）和大肠杆菌（E.颗粒菌（Eg.）此外，据报道，具有来自肝片吸虫（Fh）的该序列的化合物被具有Eg感染的血清识别。我们的目的是调查这个序列是否是在许多种寄生虫的广泛常见的表位之一。对于各种寄生虫，Fh感染的血清对Em抗原的交叉反应频率最高（71中心点4%）。在其他寄生虫感染的患者中，血清显示针对结合至Em抗原的Fh抗原的交叉反应，具有高频率（23个中心点7%）。与商业Gal β 1- 6 Gal二糖的结合抑制试验表明，Gal β 1- 6 Gal不仅是Em、Eg和Fh之间的共同表位，而且也是各种其他寄生虫之间的共同表位。此外，Em抗原中Gal β 1- 6 Gal表位的存在通过用该序列的特异性抗体进行免疫印迹测试来证实。这项研究表明，Gal β 1- 6 Gal序列是解释Em和各种其他寄生虫之间血清学交叉反应性的抗原表位之一。
P>Parasitic helminths express various antigenic carbohydrates, which often account for serological cross-reactions. In serodiagnosis, it is essential to inspect cross-reactivity between the target parasite and other parasites in order to assess diagnostic performance. Our previous study showed that the Gal beta 1-6Gal sequence was a common epitope between Echinococcus multilocularis (Em) and E. granulosus (Eg). Furthermore, compounds with this sequence from Fasciola hepatica (Fh) reportedly were recognized by sera with Eg infection. Our aim is to investigate whether this sequence is one of the widely common epitopes in many kinds of parasites. For various parasites, sera with Fh infection cross-reacted at the highest frequency (71 center dot 4%) against Em antigen. In patients with other parasitic infections, sera showed cross-reactions against Fh antigen bound to Em antigen with a high frequency (23 center dot 7%). Binding inhibition tests with commercial Gal beta 1-6Gal disaccharide showed that Gal beta 1-6Gal was the common epitope between not only Em, Eg and Fh, but also between various other parasites. Furthermore, the presence of the Gal beta 1-6Gal epitope in Em antigen was confirmed by immunoblot testing with the specific antibody for this sequence. This study showed that the Gal beta 1-6Gal sequence is one of the antigenic epitopes that accounts for serological cross-reactivity between Em and various other parasites.