Comparison of genotypes C and D of the hepatitis B virus in Japan: A clinical and molecular biological study
Comparison of genotypes C and D of the hepatitis B virus in Japan: A clinical and molecular biological study
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DOI：
10.1002/jmv.20044
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发表时间：
2004-04-01
影响因子：
12.7
通讯作者：
Onji, M
中科院分区：
文献类型：
--
作者：
Duong, TN;Horiike, N;Onji, M
乙型肝炎病毒 (HBV) 基因型 C 和基因型 D 之间与基因型相关的差异仍不清楚。研究了 HBV 基因型与其临床特征之间的关系，特别关注基因型 C 和 D。使用酶免疫分析 (EIA) 和限制性片段长度多态性对 413 名 HBV 携带者的血清样本进行了基因分型。通过直接测序分析基本核心启动子（BCP）和前核心（PreC）区域的核苷酸序列。还检查了三例 D 基因型 HBV 病例的全基因组序列。几乎所有 D 基因型 HBV 携带者均为无症状携带者（84.2%）。在肝硬化和肝细胞癌患者中未发现 D 基因型。相比之下，C基因型携带者主要患有慢性肝病（63.2%；P < 0.001）。青年匹配组中C基因型乙型肝炎e抗原（HBeAg）/抗HBe比值显着高于D基因型（P < 0.01）。 HBeAg 阴性患者中，D 基因型 BCP（T1762、A1764）突变显着低于 C 基因型（P < 0.05）。 HBV 全基因组序列与来自欧洲的某些 D 基因型 HBV 序列非常相似。总之，C 基因型与慢性肝病相关，而 D 基因型与早期 HBeAg 血清转化的无症状携带者相关。因此，慢性 HBV 感染的结果在感染 HBV 基因型 C 和 D 的人中可能会有所不同。 (C) 2004 Wiley-Liss, Inc.
The genotype-related differences between genotype C and genotype D of the hepatitis B virus (HBV) remain unknown. The relationship was studied between the HBV genotypes and their clinical features, paying special attention to genotypes C and D. Serum samples from 413 HBV carriers were genotyped using an enzyme immunoassay (EIA) and by restriction fragment length polymorphism. The nucleotide sequences at the basic core promoter (BCP) and precore (PreC) regions were analysed by direct sequencing. The full genome sequences of three HBV genotype D cases were also examined. Almost all carriers with HBV genotype D were asymptomatic carriers (84.2%). Genotype D was not found in patients with liver cirrhosis and hepatocellular carcinoma. In contrast, carriers with genotype C had mainly chronic liver disease (63.2%; P < 0.001). The ratio of hepatitis B e antigen (HBeAg)/anti-HBe was significantly higher in genotype C than in genotype D in the young age-matched group (P < 0.01). The mutation at BCP (T1762, A1764) was significantly lower in genotype D than in genotype C among HBeAg-negative patients (P < 0.05). The HBV full-genome sequences are very similar to certain HBV genotype D sequences from Europe. In conclusion, genotype C was associated with chronic liver disease, whereas genotype D was related to asymptomatic carriers with earlier HBeAg seroconversion. Thus, the outcome of chronic HBV infection may be different in persons infected with HBV genotypes C and D. (C) 2004 Wiley-Liss, Inc.