Novel multi-component enzyme machinery in lactic acid bacteria catalyzing C=C double bond migration useful for conjugated fatty acid synthesis
Novel multi-component enzyme machinery in lactic acid bacteria catalyzing C=C double bond migration useful for conjugated fatty acid synthesis
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DOI：
10.1016/j.bbrc.2011.11.022
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发表时间：
2011-12-09
影响因子：
3.1
通讯作者：
Ogawa, Jun
中科院分区：
文献类型：
--
作者：
Kishino, Shigenobu;Park, Si-Bum;Ogawa, Jun
植物乳杆菌亚油酸异构酶是一种由三种酶组成的多组分体系，它们同时存在于植物乳杆菌的膜和可溶性部分中。膜组分中存在一种酶(CLA-HY)，而可溶性组分中存在两种酶(CLA-DH和CIA-DC)。表达CLA-HY的三个大肠杆菌转化子。构建了CLA-Dh和CIA-DC。共轭亚油酸(CIA)和10-羟基-12-十八烯酸(10-羟基-12-十八碳烯酸)只有在这三种转化子同时作为催化剂的情况下才能从亚油酸中合成。CLA-HY催化亚油酸异构化过程中的水合反应生成10-羟基-12-十八碳烯酸。这种多组分的酶系统需要NADH和FAD等氧化还原辅因子。这是首次揭示乳酸菌中的酶基因和合成CIA的复杂机制，特别是顺-9的重要异构体--反式-11-CLA。(C)2011 Elsevier Inc.保留所有权利。
Linoleic acid isomerase was identified as a multi-component enzyme system that consists of three enzymes that exist in both the membrane and soluble fractions of Lactobacillus plantarum. One enzyme (CLA-HY) is present in the membrane fraction, while two enzymes (CLA-DH and CIA-DC) exist in the soluble fraction. Three Escherichia coli transformants expressing CLA-HY. CLA-DH, and CIA-DC were constructed. Conjugated linoleic acid (CIA) and 10-hydroxy-12-octadecenoic acid were generated from linoleic acid only when all these three E. coli transformants were used as catalysts simultaneously. CLA-HY catalyzed the hydration reaction, a part of linoleic acid isomerization, to produce 10-hydroxy-12-octadecenoic acid. This multi-component enzyme system required oxidoreduction cofactors such as NADH and FAD. This is the first report to reveal enzymes genes and the elaborate machinery that synthesizes CIA, especially an important isomer of cis-9, trans-11-CLA, in lactic acid bacteria. (C) 2011 Elsevier Inc. All rights reserved.