Protective effect of low molecular weight polyethylene glycol on the repair of experimentally damaged neural membranes in rat's spinal cord
Protective effect of low molecular weight polyethylene glycol on the repair of experimentally damaged neural membranes in rat's spinal cord
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DOI：
10.1179/1743132812y.0000000133
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发表时间：
2013-05-01
影响因子：
1.9
通讯作者：
Mobasheri, Hamid
中科院分区：
文献类型：
--
作者：
Kouhzaei, Sogolie;Rad, Iman;Mobasheri, Hamid
目的：膜破坏是导致脊髓损伤（SCI）中轴突损伤和功能缺陷的主要因素之一。在本研究中，我们使用聚乙二醇 (PEG) 诱导脊髓膜立即封闭并促进 SCI 后的功能恢复。 方法：在大鼠 SCI 模型中，通过脊髓诱发电位 (SCEP) 监测 PEG (200-2000 Da) 对受损膜的影响。在一项平行研究中，神经细胞膜在培养物中被超声波（20 kHz）机械损伤，随后在单细胞水平上检测了 PEG 的修复效果。结果：SCEP 分析表明，PEG 越小，SCEP 的最终回收率越高（即 200 Da 导致 49.5%，2000 Da 高达 16.3%）。当损伤脊髓用PEG200处理25分钟时，恢复率最大，呈多项式趋势。通过MTT法对培养物中机械损伤细胞的存活率进行分析，结果表明，PEG越小，膜封闭率越高； PEG400 (20% w/w) 为 77.8 +/- 3.5，而 PEG2000 (20% w/w) 为 32.1 +/- 6.9。大的（PEG1000和2000）在低浓度时表现出轻微的修复作用，在高浓度（>50%）时没有表现出明显的密封效果。结论：我们的研究表明，低分子量PEG的应用，（
Objectives: Membrane disruption is one of the main factors that cause axonal damage and functional deficits manifested in spinal cord injury (SCI). In this study, we used polyethylene glycol (PEG) to induce immediate membrane sealing and to promote functional recovery after SCI.Methods: The effects of PEG (200-2000 Da) on the damaged membrane were monitored by means of spinal cord evoked potentials (SCEPs) in an SCI model in rats. In a parallel study, membranes of neural cells were mechanically damaged in culture by ultrasound waves (20 kHz) and the repairing effects of PEGs were examined afterwards at the single cell level.Results: Analysis of SCEPs showed that the smaller the PEG, the higher was the ultimate recovery of SCEP (i.e. 200 Da caused 49.5% and 2000 Da up to 16.3%). The rate of recovery was maximum with a polynomial trend, when the damaged spinal cord was treated with PEG200 for 25 minutes.The analysis of survival rate of mechanically damaged cells in culture, measured by MTT assay, showed that again smaller PEGs, caused higher membrane sealing rate; 77.8 +/- 3.5 for PEG400 (20% w/w) vs 32.1 +/- 6.9 for PEG2000 (20% w/w). The large ones (PEG1000 and 2000) that presented minor repair at low concentration, showed no significant sealing effects at high concentrations (>50%).Conclusion: Our studies showed that the application of low molecular weight PEGs, (