C-TERMINALS ON MOTONEURONS - ELECTRON-MICROSCOPE LOCALIZATION OF CHOLINERGIC MARKERS IN ADULT-RATS AND ANTIBODY-INDUCED DEPLETION IN NEONATES
C-TERMINALS ON MOTONEURONS - ELECTRON-MICROSCOPE LOCALIZATION OF CHOLINERGIC MARKERS IN ADULT-RATS AND ANTIBODY-INDUCED DEPLETION IN NEONATES
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DOI：
10.1016/0306-4522(94)00511-3
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发表时间：
1995-04-01
期刊：
影响因子：
3.3
通讯作者：
NAGY, JI
中科院分区：
文献类型：
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作者：
LI, W;OCHALSKI, PAY;NAGY, JI
运动神经元上的c -末端被定义为伴有特征性的突触后特化，称为表面下池。我们之前已经通过光学显微镜免疫标记方法表明，在运动神经元上含有胆碱乙酰转移酶和乙酰胆碱酯酶的钮扣下，有规律地发生地下池。在本研究中，通过免疫组织化学和电镜进一步研究了这些结果提示的c -末端的胆碱能性质，在成年大鼠和用小鼠单克隆乙酰胆碱酯酶抗体治疗的新生儿中，该抗体先前被证明会引起中枢胆碱能系统的免疫病变。在成年大鼠脊髓面核和腰椎节段，c端可见强烈的胆碱能标记物胆碱乙酰转移酶和乙酰胆碱酯酶的免疫染色。免疫标记的末梢与神经元体或大口径树突接触，胆碱能标记阳性，并表现出棍棒状或细长的形态，提示末梢或细胞传递型突触相互作用。在8日龄大鼠运动神经元上，胆碱能标记物与成人免疫标记的地下池之间的密切关系得以维持。虽然在出生后第8天检查时，用乙酰胆碱酯酶抗体皮下治疗新生大鼠似乎对运动神经元中免疫阳性的表面下池的分布没有影响，但这些神经元周围两种胆碱能标记物的标记密度降低了。在接受治疗的动物中，运动神经元周围的神经细胞区域经常显示出广泛肿胀和恶化的迹象，表明病变事件，这些运动神经元经常显示出与c端无关的地下池。这些结果支持了我们之前基于光镜研究的结论，即大多数（如果不是全部）c -末端在研究区域具有胆碱能，并证明了免疫方法在研究c -末端功能方面的潜在效用。
C-terminals on motoneurons are defined as those accompanied by characteristic postsynaptic specializations termed subsurface cisterns. We have previously shown, by light microscope immunolabelling methods, that subsurface cisterns occur regularly beneath choline acetyltransferase- and acetylcholinesterase-containing boutons on motoneurons. In the present study, the cholinergic nature of C-terminals suggested by these results was further investigated by immunohistochemistry and electron microscopy in adult rats and in neonates treated with a murine monoclonal acetylcholinesterase antibody which was previously shown to cause immunological lesions of central cholinergic systems. In both the facial nucleus and lumbar segment of spinal cord of adult rats, C-terminals were seen intensely immunostained for the cholinergic markers choline acetyltransferase and acetylcholinesterase. Immunolabelled terminals made contact with either neuronal somata or large calibre dendrites, which were positive for the cholinergic markers, and exhibited club-shaped or thin elongated morphologies suggestive of terminal or ell passant type synaptic interactions. The close relationship found between cholinergic markers and immunolabelled subsurface cisterns in adults was maintained on motoneurons of eight-day-old rats. While subcutaneous treatment of newborn rat with acetylcholinesterase antibody appeared to have no effect on the distribution of immunopositive subsurface cisterns in motoneurons when examined on postnatal day 8, the density of labelling for the two cholinergic markers around these neurons was reduced. Areas of neuropil immediately surrounding motoneurons in treated animals often showed signs of extensive swelling and deterioration indicative of a lesion event, and these motoneurons frequently displayed subsurface cisterns unapposed to C-terminals.These results support our earlier conclusion, based on light microscope investigation, that the majority if not all C-terminals are cholinergic in the areas investigated and demonstrate the potential utility of immunolesion methods in the study of C-terminal function.