Interplay between the MexA-MexB-OprM multidrug efflux system and the outer membrane barrier in the multiple antibiotic resistance of Pseudomonas aeruginosa
Interplay between the MexA-MexB-OprM multidrug efflux system and the outer membrane barrier in the multiple antibiotic resistance of Pseudomonas aeruginosa
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DOI：
10.1093/jac/45.4.433
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发表时间：
2000-04-01
影响因子：
5.2
通讯作者：
Poole, K
中科院分区：
文献类型：
--
作者：
Li, XZ;Zhang, L;Poole, K
MexAB-OprM是铜绿假单胞菌组成表达的多药外排系统，利用等基因泵突变体，通过评估泵失活和外膜渗透对抗生素敏感性的影响，评估MexAB-OprM外排泵和外膜屏障对多种抗生素耐药的贡献。泵失活和外膜通透性增加都增强了抗生素的敏感性，尽管当两者结合时达到最大的敏感性。因此，抑制抗生素外排泵和外膜渗透是逆转这种生物的抗生素耐药性的有效方法。
MexAB-OprM is a constitutively expressed multidrug efflux system of Pseudomonas aeruginosa, Using isogenic pump mutants, the contributions of the MexAB-OprM efflux pump and the outer membrane barrier to multiple antibiotic resistance were evaluated by assessing the influence of pump inactivation and outer membrane permeabilization on antibiotic susceptibility. Both pump inactivation and increased outer membrane permeability enhanced antibiotic susceptibility, although maximal susceptibility was achieved when the two were combined. Thus, inhibition of antibiotic efflux pumps and permeabilization of the outer membrane constitute an effective approach to reversing the antibiotic resistance of this organism.