A quick, easy, cheap, effective, rugged, and safe sample pretreatment and liquid chromatography with tandem mass spectrometry method for the simultaneous quantification of 33 mycotoxins in Lentinula edodes.
A quick, easy, cheap, effective, rugged, and safe sample pretreatment and liquid chromatography with tandem mass spectrometry method for the simultaneous quantification of 33 mycotoxins in Lentinula edodes.
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DOI：
10.1002/jssc.201400329
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发表时间：
2014-08
影响因子：
3.1
通讯作者：
Z. Han;Z. Feng;W. Shi;Zhihui Zhao;Yongjiang Wu;Aibo Wu
中科院分区：
文献类型：
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作者：
Z. Han;Z. Feng;W. Shi;Zhihui Zhao;Yongjiang Wu;Aibo Wu
香菇是世界上栽培量最大的食用菌之一，但由于最初认为其对霉菌具有抗性，因此真菌毒素污染问题往往被忽视。本研究建立了一种灵敏可靠的液相色谱-串联质谱法同时测定L. edodes。采用快速、简便、廉价、有效、坚固且安全的方法提取目标真菌毒素，无需任何进一步的净化步骤，并在Agilent Poroshell 120 EC-C18色谱柱（100 × 3 mm，2.7 μm）上采用液相色谱串联质谱法进行分析，采用线性梯度洗脱程序，使用含5 mM乙酸铵和甲醇的水作为移动的相。经线性（R（2）> 0.99）、灵敏度（定量限≤ 20 ng/kg）、回收率（73.6-117.9%）和精密度（0.8-19.5%）验证，该方法已成功地用于L. edodes。在30份检测样品中，22份样品被各种真菌毒素污染，浓度范围为3.3- 28，850.7 μg/kg，预测食用菌可能被产真菌毒素真菌感染。据我们所知，这是第一个关于真实的真菌毒素污染的报道。edodes。
Lentinula edodes, one of the most cultivated edible fungi in the world, are usually neglected for mycotoxins contamination due to the initial thinking of its resistance to mycotoxingenic molds. In the present study, a sensitive and reliable liquid chromatography with tandem mass spectrometry method was developed for the simultaneous quantification of 33 mycotoxins in L. edodes. Targeted mycotoxins were extracted using a quick, easy, cheap, effective, rugged, and safe procedure without any further clean-up step, and analyzed by liquid chromatography with tandem mass spectrometry on an Agilent Poroshell 120 EC-C18 column (100 × 3 mm, 2.7 μm) with a linear gradient elution program using water containing 5 mM ammonium acetate and methanol as the mobile phase. After validation by determining linearity (R(2) > 0.99), sensitivity (LOQ ≤ 20 ng/kg), recovery (73.6-117.9%), and precision (0.8-19.5%), the established method has been successfully applied to reveal the contamination states of various mycotoxins in L. edodes. Among the 30 tested samples, 22 were contaminated by various mycotoxins with the concentration levels ranging from 3.3-28,850.7 μg/kg, predicting that the edible fungus could be infected by the mycotoxins-producing fungi. To the best of our knowledge, this is the first report about real mycotoxins contamination in L. edodes.