Serum high-temperature-required protein A2: A potential biomarker for the diagnosis of breast cancer
Serum high-temperature-required protein A2: A potential biomarker for the diagnosis of breast cancer
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DOI：
10.1016/j.genrep.2020.100706
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发表时间：
2020-09-01
期刊：
影响因子：
1.3
通讯作者：
Mariah, Reham A.
中科院分区：
文献类型：
--
作者：
Ghafar, Muhammad Tarek Abdel;Gharib, Fatma;Mariah, Reham A.
背景：乳腺癌的诊断具有挑战性，因为放射学方法和肿瘤标志物如糖类抗原15-3(CA15-3)和癌胚抗原(CEA)的诊断效率有限。目的：探讨循环高温必需蛋白A2(HtrA2)基因表达在乳腺癌诊断中的价值，并探讨其与乳腺癌患者临床病理特征的关系。方法：采用逆转录聚合酶链式反应技术检测31例乳腺癌患者和28例健康女性血清中HtrA2基因的表达水平。结果：乳腺癌患者血清中HtrA2基因表达水平明显低于正常对照组(p<；0.001)。HtrA2表达降低与低、中分化程度、远处转移、淋巴结转移及临床分期有关。HtrA2诊断乳腺癌的敏感性为0.868，特异性为77.4%，特异性为85.7%。血清HtrA2与CA15-3、CEA呈负相关，提示这些指标之间存在显著的相关性。HtrA2、CA15-3、CEA联合检测诊断乳腺癌的准确率为0.947，敏感性为83.9%，特异性为96.4%。结论：htrA2是一种潜在的乳腺癌血清学诊断指标。HtrA2与低组织学分级、淋巴结转移、远处转移和晚期TNM分期相关。HtrA2与CA15-3、CEA联合检测可提高其诊断能力。
Background: Breast cancer diagnosis is challenging because of the limited diagnostic efficiency of radiological measures and tumor markers such as carbohydrate antigen 15-3 (CA15-3) and carcinoembryonic antigen (CEA). Hence, diagnostic markers with acceptable accuracy are needed for breast cancer diagnosis.Objectives: This study was performed to assess the diagnostic power of circulating high-temperature-required protein A2 (HtrA2) mRNA expression in breast cancer detection and correlate its serum level with the clinicopathological characteristics of breast cancer patients.Methods: The HtrA2 mRNA expression was measured by reverse transcription-polymerase chain reaction (qRT-PCR) in the serum of 31 breast cancer patients and 28 healthy females.Results: The HtrA2 mRNA expression was significantly down-regulated in the serum of breast cancer patients compared to healthy controls (p < 0.001). Decreased HtrA2 expression was associated with poorly and moderately-differentiated tumor histological grade, distant metastasis, lymph node metastasis (N2), and TNM stages III and IV. The diagnostic power of HtrA2 was 0.868 with sensitivity and specificity of 77.4% and 85.7% respectively for the detection of breast cancer. Serum HtrA2 was negatively correlated with CA15-3 and CEA; this negative correlation revealed a significant relationship between these markers. The diagnostic power of combined HtrA2, CA15-3, and CEA was 0.947 with sensitivity and specificity of 83.9% and 96.4% respectively.Conclusions: HtrA2 is a potential serological diagnostic marker for breast cancer. HtrA2 is found to be correlated with poor histological grade, lymph node infiltration, distant metastasis, and advanced TNM stages. Combined detection of HtrA2 with CA15-3 and CEA could increase the diagnostic power of these markers.