Effects of Citrus Auraptene (7-Geranyloxycoumarin) on Hepatic Lipid Metabolism in Vitro and in Vivo
Effects of Citrus Auraptene (7-Geranyloxycoumarin) on Hepatic Lipid Metabolism in Vitro and in Vivo
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DOI：
10.1021/jf1020329
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发表时间：
2010-08-25
影响因子：
6.1
通讯作者：
Yanagita, Teruyoshi
中科院分区：
文献类型：
--
作者：
Nagao, Koji;Yamano, Naomi;Yanagita, Teruyoshi
近年来的研究表明，柑橘金环烯(7-香叶酰氧基香豆素)具有重要的药理作用，包括抗癌、抗炎、抗螺杆菌、抗遗传毒性和神经保护作用。在本研究中，我们在体外和体内研究了饮食中的极光烯对肝脏脂质代谢的影响。结果提示，金雀烯具有纠正HepG2肝细胞脂质异常的作用。4周后，OLETF大鼠腹部白色脂肪组织重量和肝脏甘油三酯(TG)水平呈剂量依赖性降低。OLETF大鼠肝脏线粒体脂肪酸β氧化关键酶肉碱棕榈酰转移酶和过氧化物酶β氧化活性显著增强，且呈剂量依赖关系。此外，肝脏中过氧化物酶-β氧化系统的起始酶--乙酰辅酶A氧化酶的表达显著增强，且呈剂量依赖关系。这些结果提示，给药后可部分通过促进肥胖OLETF大鼠肝脏的脂解作用而减轻肥胖和肝脏TG蓄积。
Recent reports have shown that citrus auraptene (7-geranyloxycoumarin) possesses valuable pharmacological properties, including anticarcinogenic, anti-inflammatory, antihelicobacter, antigenotoxic, and neuroprotective effects. In the present study, we investigated the effect of dietary auraptene on hepatic lipid metabolism both in vitro and in vivo. Results suggested that auraptene has the ability to normalize lipid abnormalities in HepG2 hepatocytes. After 4 weeks of auraptene feeding, abdominal white adipose tissue weight and hepatic triglyceride (TG) levels were dose-dependently lowered in Otsuka Long-Evans Tokushima fatty (OLETF) rats. The activities of carnitine palmitoyltransferase, a key enzyme in mitochondrial fatty acid beta-oxidation, and peroxisomal beta-oxidation were markedly and dose-dependently enhanced in OLETF rat livers by auraptene feeding. Additionally, hepatic expression of acyl-CoA oxidase, the initial enzyme of the peroxisomal beta-oxidation system, was significantly and dose-dependently enhanced by auraptene administration. These results suggest that auraptene administration alleviates obesity and hepatic TG accumulation in part through lipolysis enhancement in the livers of obese OLETF rats.