OVA-specific CD8+T cells do not express granzyme B during anterior chamber associated immune deviation
OVA-specific CD8+T cells do not express granzyme B during anterior chamber associated immune deviation
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DOI：
10.1007/s00417-006-0255-0
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发表时间：
2006-03
期刊：
影响因子：
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通讯作者：
Y. Ren;Peizeng Yang;Bing Li;Yang Gao;Hong-yan Zhou;Xiang-kun Huang;Lian-xiang Zhu;A. Kijlstra
中科院分区：
文献类型：
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作者：
Y. Ren;Peizeng Yang;Bing Li;Yang Gao;Hong-yan Zhou;Xiang-kun Huang;Lian-xiang Zhu;A. Kijlstra
目的探讨前房相关免疫偏离（ACAID）时抗原（Ag）特异性CTL反应。方法将sova或OVA257-264肽注入C57BL/6小鼠前房（AC）。用OVA或OVA257-264肽诱导ACAID组各16只小鼠。小鼠于第7天用SC注射OVA或OVA 257-264肽作为完全弗氏佐剂（CFA）。第14天使用Pentamer H-2Kb-SIINFEKL（OVA257-264肽）分析脾脏ag特异性CD8+T细胞。使用IFN-γ ELISPOT和细胞内颗粒酶B染色来表征CTL反应。每组12只小鼠分别免疫OVA或CFA中OVA257-264肽作为阳性对照。12只正常小鼠作为阴性对照，12只注射CFA作为CFA对照，研究CFA对ag特异性CTL反应的影响。结果前房接种OVA或OVA257-264肽可诱导ACAID， DTH反应受损。ACAID小鼠中ag特异性CD8+T细胞的频率与仅在CFA（阳性对照）中受到Ags攻击的小鼠没有差异。与阳性对照相比，ACAID小鼠中这些细胞产生的IFN-γ没有差异。然而，ACAID小鼠的ag特异性CD8+T细胞不能分泌颗粒酶B。仅用OVA肽和CFA攻毒的小鼠也表现出颗粒酶B阴性的CD8+T细胞反应。单独使用CFA诱导的ag特异性CTL反应与阴性对照组相似。结论ACAID期间ag特异性CD8+T细胞频率未发生改变。ACAID期间ag特异性CTL反应的特征是缺乏颗粒酶B的表达。
PurposeTo examine antigen (Ag)-specific CTL response during anterior chamber associated immune deviation (ACAID).MethodsOVA or OVA257-264 peptide was injected into the anterior chamber (AC) of C57BL/6 mice. There were 16 mice in each ACAID group induced with OVA or OVA257-264 peptide. The mice were primed by SC injection with OVA or OVA 257-264 peptide in complete Freund’s adjuvant (CFA) on day 7. Ag-specific CD8+T cells in spleens were analyzed on day 14 using Pentamer H-2Kb-SIINFEKL(OVA257-264 peptide). IFN-γ ELISPOT and intracellular granzyme B staining were used to characterize the CTL response. Twelve mice in each group immunized with OVA or OVA257-264 peptide in CFA served as positive controls. Twelve normal mice served as negative controls and 12 receiving injection of CFA as CFA controls for studying the influence of CFA on the Ag-specific CTL response.ResultThe results showed that anterior chamber inoculation of OVA or OVA257-264 peptide could induce ACAID as evidenced by an impaired DTH response. The frequency of Ag-specific CD8+T cells in ACAID mice was not different from that in mice challenged with Ags in CFA only (positive controls). IFN-γ production by these cells in ACAID mice was not different compared to positive controls. However, Ag-specific CD8+T cells in ACAID mice failed to secrete granzyme B. Mice challenged only with OVA peptide and CFA also showed a granzyme B negative CD8+T cell response. Ag-specific CTL response induced by CFA alone was similar with the negative control.ConclusionThese results show that the frequency of Ag-specific CD8+T cells is not altered during ACAID. The Ag-specific CTL response during ACAID is characterized by the absence of granzyme B expression.