Efficient differentiation of cardiomyocytes from human pluripotent stem cells with growth factors.
Efficient differentiation of cardiomyocytes from human pluripotent stem cells with growth factors.
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DOI：
10.1007/978-1-4939-2572-8_9
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Xu C
中科院分区：
文献类型：
--
作者：
Jha R;Xu RH;Xu C
Human pluripotent stem cells have tremendous replicative capacity and demonstrated potential to generate functional cardiomyocytes. These cardiomyocytes represent a promising source for cell replacement therapy to treat heart disease and may serve as a useful tool for drug discovery and disease modeling. Efficient cardiomyocyte differentiation, a prerequisite for the application of stem cell-derived cardiomyocytes, can be achieved with a growth factor-guided method. Undifferentiated cells are sequentially treated with activin A and BMP4 in a serum-free and insulin-free medium and then maintained in a serum-free medium with insulin. This method yields as much as >75% cardiomyocytes in the differentiation culture within 2 weeks, and the beating cardiomyocytes have expected molecular, cellular and electrophysiological characteristics. In this chapter, we describe in detail the differentiation protocol and follow-up characterization focusing on immunocytochemistry, quantitative RT-PCR and flow cytometry analysis.
