Developmental regulation of vesicle transport in Drosophila embryos:: Forces and kinetics
Developmental regulation of vesicle transport in Drosophila embryos:: Forces and kinetics
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DOI：
10.1016/s0092-8674(00)80947-2
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发表时间：
1998-02-20
期刊：
影响因子：
64.5
通讯作者：
Wieschaus, EF
中科院分区：
文献类型：
--
作者：
Welte, MA;Gross, SP;Wieschaus, EF
在果蝇胚胎中，沿顶端-底端方向定向的微管支持跳跃性小泡运动。囊泡运输包括脂滴，其分布在早期胚胎发生两次转移。使用显微镜、光钳和一种新型的压装胚胎制备方法，我们跟踪了单个液滴并测量了这些液滴产生的力。液滴失速力以大致量化的方式发展变化，与活跃马达数量的变化一致。我们鉴定了一种突变，klarsicht，它会影响液滴的运输。KLAR(+)促进了力量的变化，可能是通过协调多个马达的活动。运输的改变影响了心尖和心尖方向的运动，表明了相反极性的电机之间的紧密耦合。KLAR基因的突变也会影响眼睛发育过程中的核迁移，这表明基于KLAR的运输具有多种作用。
In Drosophila embryos, microtubules oriented along apical-basal directions support saltatory vesicle movement. Vesicle traffic includes lipid droplets whose distribution shifts twice during early embryogenesis. Using microscopy, optical tweezers, and a novel squashed-mount embryo preparation, we tracked single droplets and measured the forces these generated. Droplet stalling forces change developmentally, in a roughly quantized fashion, consistent with variation in the number of active motors. We characterized a mutation, klarsicht, that affects droplet transport. Klar(+) facilitates changes in force, possibly by coordinating the activity of multiple motors. Alterations in transport affected motion in both apical and basal directions, indicating tight coupling between motors of opposite polarity. Mutations in klar also affect nuclear migration during eye development, suggesting multiple roles for klar-based transport.