Change in IgG1 Fc N-linked glycosylation in human lung cancer: Age- and sex-related diagnostic potential
Change in IgG1 Fc N-linked glycosylation in human lung cancer: Age- and sex-related diagnostic potential
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DOI：
10.1002/elps.201200455
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发表时间：
2013-08-01
期刊：
影响因子：
2.9
通讯作者：
Li, Zhili
中科院分区：
文献类型：
--
作者：
Chen, Guoqiang;Wang, Yanmin;Li, Zhili
IgG Fc区与Fc受体的相互作用通过在Asn-297处的单一保守N-连接糖基化位点的定制来优化。我们的前期研究表明，与年龄相关的Fc-糖基化变化具有性别特异性，并且Fc-糖基化具有疾病区分的潜力。在这里，我们进行了傅立叶变换离子回旋共振MS为基础的分析研究，涉及410名对照组和259名肺癌（LC）患者。与健康对照组相比，LC患者IgG（1）Fc-无半乳糖基化显著增加，半乳糖基化降低。采用二元Logistic回归结合受试者工作特征曲线确定IgG（1）Fc-糖基化的诊断能力。结果发现，这种诊断能力是性别和年龄依赖。此外，对多种不同生理和病理条件下Fc-糖基化的变化进行了回顾性讨论。这些数据进一步理解了人类LC中的免疫相关变化，并且也可能在将来尝试Fc-糖基化相关的诊断评估和临床测定中有用。
The interactions of IgG Fc region with Fc receptors are optimized by the tailoring of a single-conserved N-linked glycosylation site at Asn-297. Our previous study has demonstrated that the age-related Fc-glycosylation change is featured by sex specificity and that the Fc-glycosylation has the potential for disease discrimination. Here, we conducted a Fourier transform ion cyclotron resonance MS-based profiling study involving 410 control individuals and 259 lung cancer (LC) patients. As compared to healthy controls, the marked increase in IgG(1) Fc-agalactosylation and decrease in galactosylation were observed in LC patients. The binary logistic regression in combination with the receiver operating characteristic curve was used to determine the diagnostic ability of IgG(1) Fc-glycosylation. It was found that this diagnostic ability was both sex and age dependent. Additionally, the change in Fc-glycosylation upon many different physiological and pathological conditions was retrospectively discussed. The data furthered the understanding of the immune-associated change in human LC, and also might be useful in the future attempts for Fc-glycosylation-associated diagnostic evaluations and clinical assays.