Postnatal development of bitter taste avoidance behavior in mice is associated with ACTIN-dependent localization of bitter taste receptors to the microvilli of taste cells
Postnatal development of bitter taste avoidance behavior in mice is associated with ACTIN-dependent localization of bitter taste receptors to the microvilli of taste cells
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小鼠出生后苦味回避行为的发展与肌动蛋白依赖性苦味受体定位到味觉细胞微绒毛有关
DOI：
10.1016/j.bbrc.2017.12.126
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Ota MS.
中科院分区：
文献类型：
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作者：
Yamashita A;Kondo K;Kunishima Y;Iseki S;Kondo T;Ota MS.
苦味回避行为（BAB）在对有毒物质的苦味回避中起着重要的作用。然而，BAB发展的分子基础尚不清楚。为了研究味蕾转变为具有BAB功能器官的关键发育事件，我们研究了出生后小鼠对苦味化合物（如奎宁和硫胺素）的早期BAB发育。出生后小鼠分别在出生后第5天（PD5）和第7天开始对硫胺素和奎宁产生BAB。味蕾的组织学分析显示，味觉孔中的微绒毛形成始于PD5, 2型味觉受体119 （TAS2R119）定位于PD6的微绒毛。用细胞松弛素D （CytD）处理舌头上皮，会干扰微绒毛中的ACTIN聚合，导致TAS2R119在微绒毛中的定位丢失，奎宁和硫胺素的BAB丢失。在CytD治疗后，味觉刺激引起的环腭乳头组织ATP释放也有所下降。这些结果表明TAS2R119在味觉微绒毛上的定位对BAB的发生至关重要。
Bitter taste avoidance behavior (BAB) plays a fundamental role in the avoidance of toxic substances with a bitter taste. However, the molecular basis underlying the development of BAB is unknown. To study critical developmental events by which taste buds turn into functional organs with BAB, we investigated the early phase development of BAB in postnatal mice in response to bitter-tasting compounds, such as quinine and thiamine. Postnatal mice started to exhibit BAB for thiamine and quinine at postnatal day 5 (PD5) and PD7, respectively. Histological analyses of taste buds revealed the formation of microvilli in the taste pores starting at PD5 and the localization of type 2 taste receptor 119 (TAS2R119) at the microvilli at PD6. Treatment of the tongue epithelium with cytochalasin D (CytD), which disturbs ACTIN polymerization in the microvilli, resulted in the loss of TAS2R119 localization at the microvilli and the loss of BAB for quinine and thiamine. The release of ATP from the circumvallate papillae tissue due to taste stimuli was also declined following CytD treatment. These results suggest that the localization of TAS2R119 at the microvilli of taste pores is critical for the initiation of BAB.