Protective Effects of Enteral Nutrition Supplemented with Crassostrea hongkongensis Polysaccharides Against 5-Fluorouracil-Induced Intestinal Mucosal Damage in Rats
Protective Effects of Enteral Nutrition Supplemented with Crassostrea hongkongensis Polysaccharides Against 5-Fluorouracil-Induced Intestinal Mucosal Damage in Rats
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DOI：
10.1089/jmf.2017.4025
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发表时间：
2018-02-12
影响因子：
2.4
通讯作者：
Pan, Jianyu
中科院分区：
文献类型：
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作者：
Cai, Bingna;Wan, Peng;Pan, Jianyu
化疗药物，包括5-氟尿嘧啶（5-FU），经常损害肠粘膜屏障，引起肠粘膜炎（IM）。补充来自香港牡蛎的免疫调节多糖已被证明对5-FU的有效性和毒性有积极影响。因此，我们研究了牡蛎多糖对5-FU诱导的大鼠肠粘膜损伤的影响。C30-60%乙醇沉淀的多糖组分促进IEC-6细胞增殖，并在0.0195 mg/mL浓度下表现出最大效果。此外，以C30-60%多糖为基础的营养配方（OPNF）治疗部分防止5-FU诱导的小肠粘膜组织学和超微结构的退行性变化。此外，5-FU和OPNF组大鼠的内毒素水平下降至正常对照组水平。此外，5-FU诱导的外周血中促炎细胞因子白细胞介素（IL）-2的升高和抗炎细胞因子IL-10水平的降低被OPNF给药显著减弱。结论：牡蛎C30-60%多糖可部分预防5-FU诱导的IM，减轻炎症，促进免疫。
Chemotherapeutics, including 5-fluorouracil (5-FU), often damage the intestinal mucosal barrier and cause intestinal mucositis (IM). Supplementation with immunoregulatory polysaccharides from Crassostrea hongkongensis has been shown to positively influence the effectiveness and toxicity of 5-FU. Therefore, we studied the effects of oyster polysaccharides on 5-FU-induced intestinal mucosal damage in rats. The C30-60% ethanol-precipitated fraction of polysaccharides promoted IEC-6 cell proliferation and exhibited a maximal effect at a 0.0195 mg/mL concentration. Moreover, treatment with C30-60% polysaccharide-based nutrition formula (OPNF) partially prevented the 5-FU-induced degenerative changes in the histology and ultrastructure of small intestinal mucosa. In addition, the endotoxin level of rats fed with 5-FU and OPNF decreased to the normal control level. Furthermore, the 5-FU-induced increase of proinflammatory cytokine interleukin (IL)-2 and the decrease of anti-inflammatory cytokine IL-10 level in the peripheral blood were significantly attenuated by OPNF administration. In conclusion, Oyster C30-60% polysaccharides can ameliorate 5-FU-induced IM by partially preventing mucosal damage, reducing inflammation, and promoting immunity.