Isolation, Identification and Molecular Screening of Pseudomonas sp Metabolic Pathways NRPs and PKS Associated with the Red Sea Sponge, Hyrtios aff. Erectus, Egypt
Isolation, Identification and Molecular Screening of Pseudomonas sp Metabolic Pathways NRPs and PKS Associated with the Red Sea Sponge, Hyrtios aff. Erectus, Egypt
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DOI：
10.22207/jpam.11.3.10
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发表时间：
2017-09-01
影响因子：
0.8
通讯作者：
Nabil-Adam, Asmaa
中科院分区：
文献类型：
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作者：
Abd El-Moneam, Nehad M.;El-Assar, Samy A.;Nabil-Adam, Asmaa
埃及红海的海洋沿海地区具有巨大的海绵生物多样性，其中含有大量的内共生细菌，这些细菌是许多潜在的生物活性代谢物的来源，并被认为是一个很好的来源。本研究主要是分离与海绵Hyrtios aff共生的假单胞菌。对红海直立蟹进行纯培养，并对其代谢途径聚酮酶和非核糖体肽（PSK和NRP）进行遗传学鉴定和筛选。通过使用DPPH和细胞系的抗氧化剂和细胞毒性测定来表征细菌产生的生物活性代谢物。16S rRNA基因序列分析表明，该菌株与假单胞菌属（Pseudomonassp.）假单胞菌代谢途径活性基因为PSK Ⅱ。假单胞菌的抗氧化和细胞毒性生物活性最可能与存在相关的生物活性代谢途径相关的聚酮家族II型基因有关。此外，通过定量总酚含量（TPC）和2，2-二苯基-1-苦基肼（DPPH）自由基清除活性和抗氧化活性来评价假单胞菌提取物的抗氧化活性。不同浓度（50 mg-6.25 mg）的抗氧化活性最高，其中50 mg、25 mg和12.25 mg的抗氧化能力均达到100%。从细胞毒性，抗氧化剂和生物活性代谢筛选途径，该菌株是一个很好的来源，天然抗氧化剂和细胞毒性化合物对自由基和癌细胞系。
Marine coastal areas of the Red sea in Egypt have great biodiversity of sponges which harbors a huge number of endosymbiotic bacteria which are the source of many potential bioactive metabolites and consider as an excellent source. The study focuses on the isolation of Pseudomonas sp associated with marine sponge Hyrtios aff. Erectus from the Red Sea in pure culture, phylogenetically identified and screening of their metabolic pathways polyketide synthases and Nonribosomal peptides (PSK and NRPs). Characterization of the bacterial produced bioactive metabolite was done through antioxidant and cytotoxicity assays using DPPH and cell line. The 16S rRNA gene sequencing of bacterial isolate revealed that the bacterial strain was closely related to Pseudomonas sp., and the active metabolic pathways genes in the Pseudomonas sp was PSK II. The antioxidants and cytotoxicity bioactivity of Pseudomonas sp as is most probably related to the presence of associated bioactive metabolic pathways genes related to polyketides family type II. Furthermore, the antioxidant activity of Pesudomance sp extract, performed by quantification of total phenolic content (TPC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity antioxidant activities, were evaluated. The highest antioxidant activity was exhibited at different concentrations (50 mg-6.25 mg) which showed a potent antioxidant capacity by 100% at 50mg, 25 mg and 12.25 mg. From the cytotoxicity, antioxidant and bioactive metabolic screening pathways this bacterial strain was revealed to be an excellent source of natural antioxidant and cytotoxicity compounds against free radical and cancerous cell line.