Development of the binding molecules for the RNA higher-order structures based on the guanine-recognition by the G-clamp
Development of the binding molecules for the RNA higher-order structures based on the guanine-recognition by the G-clamp
复制标题
基于G-clamp鸟嘌呤识别的RNA高级结构结合分子的开发
DOI：
10.1016/j.bmcl.2019.03.052
复制
发表时间：
2019
影响因子：
2.7
通讯作者：
Nagatsugi Fumi
中科院分区：
文献类型：
--
作者：
Murase Hirotaka;Nagatsugi Fumi
RNA的高阶结构对基因表达起着重要的调控作用，结合在这些结构上的小分子有可能在RNA介导的途径中起到干扰的作用。在本研究中，我们合成了基于G-钳结构的新的RNA结合分子，并利用模型RNA评估了它们的结合性质。单体G-钳制配体与RNA结合后荧光猝灭。二聚体G-钳配体对含有鸟嘌呤的RNA发夹结构显示出显著的荧光OFF/ON响应，表明G-钳二聚体与位于RNA发夹环中的两个相邻的鸟嘌呤具有很高的亲和力。
RNA higher-order structures play an important role for control of the gene expression, and the small molecules binding to these structures have potential to act as interfering agents in the RNA-mediated-pathway. In this study, we synthesized new RNA binding molecules based on the G-clamp structure and evaluated their binding properties using the model RNA. The monomeric G-clamp ligand exhibited a fluorescence quenching with RNA-binding. The dimeric G-clamp ligand showed a significant fluorescence OFF/ON response to the RNA hairpin structure containing the guanines, indicating a high affinity of the G-clamp dimer to two neighboring guanines located in the RNA hairpin loop.