IMMUNOHISTOCHEMICAL LOCALIZATION OF SEVERAL CYTOSKELETAL PROTEINS IN INNER-EAR SENSORY AND SUPPORTING CELLS
IMMUNOHISTOCHEMICAL LOCALIZATION OF SEVERAL CYTOSKELETAL PROTEINS IN INNER-EAR SENSORY AND SUPPORTING CELLS
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DOI：
10.1016/0378-5955(82)90082-x
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发表时间：
1982-01-01
期刊：
影响因子：
2.8
通讯作者：
WEBER, K
中科院分区：
文献类型：
--
作者：
FLOCK, A;BRETSCHER, A;WEBER, K
几个结构和收缩蛋白质进行了搜索与免疫组织化学方法，使用针对这些蛋白质的抗体。使用了来自豚鼠的三种类型的制备物：来自椭圆囊的分离的静纤毛、通过细胞解离获得的Corti器官片段和通过冷冻显微镜下获得的0.02-1 μ m切片。主要的发现是，不同的蛋白质组成的细胞骨架在支持细胞和机械感受器结构的感觉细胞。肌动蛋白被发现与毛细胞的机械感受区的fimalloy;支持细胞，虽然富含肌动蛋白，并没有显示fimalloy。相反，微管蛋白与肌动蛋白一起出现在支持细胞中，这些细胞也显示出前角蛋白。纤维蛋白似乎是一种能够从肌动蛋白丝形成纤维束和网络的蛋白质。它在感觉细胞的机械敏感区的唯一存在可能与这些细胞作为机械感受器的功能有关。
Several structural and contractile proteins were searched for with immunohistochemical methods using antibodies directed against these proteins. Three types of preparations from the guinea pig were used: isolated stereocilia from the utricle, organ of Corti fragments obtained by cellular dissociation and 0.02-1 .mu.m sections obtained by cryoultramicrotomy. The main finding is that different sets of proteins compose the cytoskeleton in supporting cells and the mechanoreceptor structures of the sensory cells. Actin was found in association with fimbrin in the mechanoreceptive region of hair cells; supporting cells, although rich in actin, did not reveal fimbrin. Instead tubulin was seen together with actin in supporting cells which also exhibited prekeratin. Fimbrin appears to function as a protein capable of making bundles and networks from actin filaments. Its exclusive presence in the mechanosensitive region of the sensory cells is possibly related to the function of these cells as mechanoreceptors.