Isolation and functional characterization in yeast of CYP72A 18, a rice cytochrome P450 that catalyzes (ω-1)-hydroxylation of the herbicide pelargonic acid
Isolation and functional characterization in yeast of CYP72A 18, a rice cytochrome P450 that catalyzes (ω-1)-hydroxylation of the herbicide pelargonic acid
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DOI：
10.1016/j.pestbp.2006.09.003
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发表时间：
2007-05-01
影响因子：
4.7
通讯作者：
Matumoto, Suzuko
中科院分区：
文献类型：
--
作者：
Imaishi, Hiromasa;Matumoto, Suzuko
细胞色素P450蛋白在植物除草剂选择性中起重要作用。在这里，我们证明了从水稻Oryza sativa L. cv中分离出来的一种新的细胞色素P450 CYP72A18对除草剂pelargonic酸的代谢作用。Nipponbare。从水稻中克隆了CYP72A18 cDNA，并通过乙醇脱氢酶（ADH1）启动子在酿酒酵母AH22细胞中异种表达。从重组酵母细胞中分离的微粒体含有CYP72A18，该蛋白被发现催化除草剂天龙酸的(omega- 1)-羟基化。我们还表明(omega- 1)-羟化氢酸降低了对水稻幼苗的除草剂活性。基于这些结果，我们认为CYP72A18参与了水稻对除草剂天龙英酸的解毒。(c) 2006爱思唯尔公司版权所有。
Cytochrome P450 proteins play important roles in plant herbicide selectivity. Here, we demonstrate metabolism of the herbicide pelargonic acid by CYP72A18, a novel cytochrome P450 isolated from the rice Oryza sativa L. cv. Nipponbare. The CYP72A18 cDNA was cloned from rice and heterologously expressed in Saccharomyces cerevisiae AH22 cells from the alcohol dehydrogenase (ADH1) promoter. Microsomes isolated from recombinant yeast cells contained the CYP72A18, which was found to catalyze the (omega-l)-hydroxylation of the herbicide pelargonic acid. We also show that (omega-l)-hydroxypelargonic acid has reduced herbicide activity against rice seedlings. Based on these results, we suggest that CYP72A18 participates in the detoxification of the herbicide pelargonic acid in rice plants. (c) 2006 Elsevier Inc. All rights reserved.