TRANSFER OF THE ESCHERICHIA-COLI O-6-METHYLGUANINE METHYLTRANSFERASE GENE INTO REPAIR-DEFICIENT HUMAN-CELLS AND RESTORATION OF CELLULAR-RESISTANCE TO N-METHYL-N'-NITRO-N-NITROSOGUANIDINE
TRANSFER OF THE ESCHERICHIA-COLI O-6-METHYLGUANINE METHYLTRANSFERASE GENE INTO REPAIR-DEFICIENT HUMAN-CELLS AND RESTORATION OF CELLULAR-RESISTANCE TO N-METHYL-N'-NITRO-N-NITROSOGUANIDINE
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DOI：
10.1016/0167-8817(86)90011-8
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发表时间：
1986-09-01
期刊：
影响因子：
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通讯作者：
IKENAGA, M
中科院分区：
文献类型：
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作者：
ISHIZAKI, K;TSUJIMURA, T;IKENAGA, M
我们构建了大肠杆菌O6-甲基鸟嘌呤-DNA甲基转移酶基因(ada基因)与SV40启动子序列和Poly(A)位点相连的表达载体。用DNA转染法将该表达载体导入Mer-HeLa MR细胞，观察到MT在大肠杆菌中的有效表达。分离的稳定转化子克隆在克隆形成和姐妹染色单体互换诱导方面对N-甲基-N‘-硝基-N-亚硝基胍的抗性高于HeLa MR细胞。
We have constructed a plasmid on which the Escherichia coli O6-methylguanine-DNA methyltransferase (MT) gene (ada gene) was linked with an SV40 promoter sequence and a poly(A) site. After transferring this plasmid into Mer- HeLa MR cells by DNA transfection, effective expression of E. coli MT was observed. Isolted stable transformant clones showed higher resistance to N-methyl-N''-nitro-N-nitrosoguanidine in colony formation and sister-chromatid exchange induction than HeLa MR cells.