Homologous muscle acellular matrix seeded with autologous myoblasts as a tissue-engineering approach to abdominal wall-defect repair
Homologous muscle acellular matrix seeded with autologous myoblasts as a tissue-engineering approach to abdominal wall-defect repair
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DOI：
10.1016/j.biomaterials.2004.07.035
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发表时间：
2005-05-01
期刊：
影响因子：
14
通讯作者：
Nussdorfer, GG
中科院分区：
文献类型：
--
作者：
Conconi, MT;De Coppi, P;Nussdorfer, GG
通过体外培养从大鼠趾短屈肌经酶消化分离出的单根肌纤维获得成肌细胞，并通过生肌分化因子、生肌因子 - 5、肌细胞生成素和结蛋白确认其表型。收集培养的成肌细胞，接种到经洗涤剂 - 酶处理腹部肌肉碎片获得的同种脱细胞基质补片上。将接种有成肌细胞的补片植入1个月大的大鼠右侧腹斜肌之间，而未接种的补片植入左侧。手术后30天，未接种的补片完全被纤维组织取代，而成肌细胞接种的补片结构在第2个月时仍保存良好。接种的补片显示有丰富的血管和成肌细胞，肌电图显示其中有单个运动单位电位，有时聚集成节律性放电。植入90天后，成肌细胞接种补片的厚度及其电活动降低，表明收缩性肌纤维缺失。总之，目前的结果表明，自体成肌细胞 - 同种脱细胞肌肉基质构建体是修复体壁缺损的一种有前景的工具，并且正在进行研究以确定能够更长时间改善和维持植入物结构和功能完整性的策略。(C) 2004爱思唯尔有限公司。保留所有权利。
Myoblasts were obtained by culturing in vitro, single muscle fibers, isolated by enzymatic digestion from rat flexor digitorum brevis, and their phenotype was confirmed by myogenic differentiation factor, myogenic factor-5, myogenin and desmin. Cultured myoblasts were harvested and seeded on patches of homologous acellular matrix, obtained by detergent-enzymatic treatment of abdominal muscle fragments. Myoblast-seeded patches were inserted between obliqui abdominis muscles on the right side of 1-month-old rats, while non-seeded patches were implanted on the left side. Thirty days after surgery, non-seeded patches were completely replaced by fibrous tissue, while the structure of myoblast-seeded patches was well preserved until the 2nd month. Seeded patches displayed abundant blood vessels and myoblasts, and electromyography evidenced in them single motor-unit potentials, sometimes grouped into arithmic discharges. Ninty days after implantation, the thickness of myoblast-seeded patches and their electric activity decreased, suggesting a loss of contractile muscle fibers. In conclusion, the present results indicate that autologous myoblast-homologous acellular muscle matrix constructs are a promising tool for body-wall defect repair, and studies are under way to identify strategies able to improve and maintain the structural and functional integrity of implants for longer periods. (C) 2004 Elsevier Ltd. All rights reserved.