A novel electrically magnetic-controllable electrochemical biosensor for the ultra sensitive and specific detection of attomolar level oral cancer-related microRNA.
A novel electrically magnetic-controllable electrochemical biosensor for the ultra sensitive and specific detection of attomolar level oral cancer-related microRNA.
复制标题
DOI：
10.1016/j.bios.2013.02.007
复制
发表时间：
2013-07
影响因子：
12.6
通讯作者：
Zongwen Wang;Jing Zhang;Ying Guo;Xiaoyan Wu;Weijuan Yang;Liangjun Xu;Jinghua Chen;Fengfu Fu
中科院分区：
文献类型：
--
作者：
Zongwen Wang;Jing Zhang;Ying Guo;Xiaoyan Wu;Weijuan Yang;Liangjun Xu;Jinghua Chen;Fengfu Fu
口腔癌的无创早期诊断是降低口腔癌死亡率的最有效手段。在本文中，我们描述了一种新型的磁可控电化学RNA生物传感器，用于超灵敏和特异性检测口腔癌相关的microRNA (miRNA)，该传感器基于自制的磁可控金电极。电控金电极结合了加热电极和磁电极的优点，具有磁场强度、方向和电极表面温度易于调节等显著优点。电磁可控金电极的优势，以及利用“连接探针”策略和基于磁珠（mb）的酶催化扩增，使得该生物传感器即使对相似mirna的检测也具有超高的灵敏度和鉴别能力。它可以检测低至0.22aM （2.2×10−19M）的口腔癌相关miRNA，回收率为93-108%，RSD<6 （n=5）。该传感器具有灵敏度高、选择性好、制作简单、操作方便、分析时间短、稳定性好、可重复使用等优点，是口腔癌早期现场诊断的理想选择。生物传感器的成功也为生物传感器在其他疾病早期诊断方面的发展带来了巨大的潜力。
Non-invasive early diagnosis of oral cancer is the most effective means to reduce mortality rate from this disease. In this paper, we described a novel magnetic-controllable electrochemical RNA biosensor for the ultra sensitive and specific detection of oral cancer-related microRNA (miRNA) based on a home-made electrically magnetic-controllable gold electrode. The electrically magnetic-controllable gold electrode combined the merits of heated electrode and magnetic electrode, has notable advantage such as that the strength and direction of the magnetic field and the temperature of the electrode's surface can be easily regulated. The advantage of electrically magnetic-controllable gold electrode, as well as the utilization of “junction-probe” strategy and magnetic beads (MBs)-based enzymatic catalysis amplification, make the biosensor has ultra-high sensitivity and discrimination ability even for the detection of similar miRNAs. It can be used to detect as low as 0.22aM (2.2×10−19M) of oral cancer-related miRNA with a recovery of 93–108% and a RSD<6 (n=5). The high sensitivity and selectivity, as well as the easiness of fabrication, operational convenience, short analysis time, good stability and re-usability, make the biosensor a promising alternative for the early point-of-care diagnosis of oral cancer. The success of the biosensor also leads to a great potential in the development of biosensor for the early diagnosis of other diseases.