Potential protective effects of oral administration of allicin on acrylamide-induced toxicity in male mice.
Potential protective effects of oral administration of allicin on acrylamide-induced toxicity in male mice.
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DOI：
10.1039/c3fo60057b
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发表时间：
2013-07
期刊：
影响因子：
6.1
通讯作者：
L. Zhang;Enting Wang;Feng Chen;Haiyang Yan;Yuan Yuan-Yuan
中科院分区：
文献类型：
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作者：
L. Zhang;Enting Wang;Feng Chen;Haiyang Yan;Yuan Yuan-Yuan
淀粉类食品在高温加热过程中会产生丙烯酰胺（AA），被认为是一种潜在的遗传毒性致癌物。然而，随着世界范围内对AA致癌性的关注，如何降低AA的毒性已成为研究热点。本研究通过测定雄性小鼠血清、肾脏、肝脏和脑中的血液学、生化和免疫学指标，进一步探讨了大蒜素对AA毒性的影响。我们的数据表明，口服大蒜素5，10和20 mg kg体重d可以显着降低硫代巴比妥反应物质（TBARS）和髓过氧化物酶（MPO）水平，同时显着增加超氧化物歧化酶（SOD）活性，谷胱甘肽S-转移酶（GST）和谷胱甘肽（GSH）水平在AA处理的小鼠的肾，肝和脑。此外，口服大蒜素不仅显著降低天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、乳酸脱氢酶（LDH）、血尿素氮（BUN）、肿瘤坏死因子α（TNF-α）、白细胞介素（IL）-1β、白细胞介素（IL）-6、活性氧（ROS）和8-羟基脱氧鸟苷（8-OHdG），但也增加了AA处理小鼠血清中的白细胞介素（IL）-10。因此，可以得出结论，口服大蒜素对AA诱导的毒性具有显著的体内保护作用。
Acrylamide (AA) forms during the heating of starchy foods at high temperature, and is regarded as a potential genotoxic carcinogen. However, with the worldwide concern about the carcinogenicity of AA, how to reduce the toxicity of AA has become a hot research topic. In this study, we further discussed the effects of oral administration of allicin on AA-induced toxicity by determining the hematological, biochemical and immunological parameters in the serum, kidney, liver, and brain of male mice. Our data showed that the orally administered allicin of 5, 10, and 20 mg kg⁻¹ bw d⁻¹ could significantly decrease thiobarbituric reactive substances (TBARS) and myeloperoxidase (MPO) levels, and simultaneously remarkably increased the superoxide dismutase (SOD) activity, glutathione S-transferase (GST) and glutathione (GSH) levels in the kidney, liver, and brain of the AA-treated mice. Furthermore, oral administration of allicin not only significantly decreased aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), blood urea nitrogen (BUN), tumor necrosis factor α (TNF-α), interleukin (IL)-1β, interleukin (IL)-6, reactive oxygen species (ROS), and 8-hydroxy-desoxyguanosine (8-OHdG), but also increased interleukin (IL)-10 in the serum of AA-treated mice. Therefore, it was concluded that oral administration of allicin had a significant in vivo protective effect against the AA induced toxicity.