Measurement of serum folate levels and serum folic acid-binding protein by 3H-PGA radioassay.
Measurement of serum folate levels and serum folic acid-binding protein by 3H-PGA radioassay.
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通过 3H-PGA 放射测定法测定血清叶酸水平和血清叶酸结合蛋白。
DOI：
10.1182/blood.v42.2.281.281
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发表时间：
1973
期刊：
影响因子：
20.3
通讯作者：
C. Schreiber
中科院分区：
文献类型：
--
作者：
S. Waxman;C. Schreiber
报道了一种使用市售β乳球蛋白作为叶酸结合蛋白和3 H-蝶酰谷氨酸（3 H-PGA）测定血清叶酸水平的放射性测定法。在室温下以一步同时添加进行测定。构建灵敏度为0.25-10 ng N-甲基四氢叶酸（甲基-THFA）的标准曲线。通过放射性测定法测量，分离为正常（大于6 ng/ml）、不确定范围（3-6 ng/ml）和缺陷（0-3 ng/ml）之间存在明显相关性。通过该检测，接受抗生素治疗的患者的血清中叶酸水平正常。3 H-PGA法测定血清叶酸结合蛋白（FABP）。在正常血清中，平均FABP为18 pg结合/0.4 ml血清，而在叶酸缺乏的血清中，平均FABP为133 pg结合/0.4 ml血清。叶酸缺乏的患者在接受叶酸治疗后，血清FABP下降到正常范围。血清脂肪酸结合蛋白，在一个病人的叶酸缺乏的饮食，增加与早期叶酸缺乏和突然下降到正常与正常饮食。叶酸缺乏骨髓细胞裂解液中FABP含量高于正常骨髓。B12缺乏、多发性骨髓瘤、肝硬化、妊娠或口服避孕药的患者FABP正常。在服用苯妥英钠的10名患者中，有2名发现FABP升高。这种放射性测定和FABP的测量应简化叶酸缺乏症的诊断。
A radioassay for the measurement of serum folate levels using commercially available beta lactoglobulin, as the folate-binding protein, and 3H-pteroylglutamic acid (3H-PGA) is reported. The assay was run in a one-step simultaneous addition at room temperature. A standard curve was constructed to a sensitivity of 0.25-10 ng of N-methyltetrahydrofolic acid (methyl-THFA). There was a clear correlation with separation into normal (greater than 6 ng/ml), indeterminate range (3-6 ng/ml), and deficient (0-3 ng/ml), as measured by radioassay. Serums from patients receiving antibiotics had normal folate levels with this assay. 3H-PGA was also used to measure serum folic acid-binding protein (FABP). In normal serums, the mean FABP was 18 pg bound/0.4 ml serum, while in folate-deficient serums it was 133 pg bound/0.4 ml of serum. Folate-deficient patients had a fall in serum FABP to the normal range when treated with folic acid. Serum FABP, in a patient on a folate-deficient diet, increased with early folate deficiency and abruptly fell to normal with a regular diet. FABP in lysates of folate-deficient bone marrow was higher than normal marrow. Patients with B12 deficiency, multiple myeloma, cirrhosis, pregnancy, or taking oral contraceptives had normal FABP. Elevated FABP was found in two out of ten patients taking Dilantin. This radioassay and the measurement of FABP should simplify the diagnosis of folate deficiency.