Alpha-particle energy loss measurement of microgram depositions of biomolecules.
Alpha-particle energy loss measurement of microgram depositions of biomolecules.
复制标题
生物分子微克沉积物的α粒子能量损失测量。
DOI：
10.1021/ac034170g
复制
发表时间：
2003
影响因子：
7.4
通讯作者：
Bench,Graham
中科院分区：
文献类型：
--
作者：
Grant,PatrickG;Palmblad,Magnus;Murov,Steven;Hillegonds,DarrenJ;Ueda,DawnL;Vogel,JohnS;Bench,Graham
A commercially available α-particle spectrometer and210Po α-particle source were used to determine the mass of microgram quantities of biomolecules. Samples were deposited in microliter volumes on thin silicon nitride windows and dried. The energy loss of the α-particles after traversing the sample was converted to a mass using tabulated α-particle stopping powers. The measurement was absolute, independent of biomolecule species, and no standards were needed for quantitation. The method has a dynamic range of 0.1−100 μg for deposits of diameter 1−2 mm. The precision varies from ∼20% at 100 ng to a few percent at 5−100 μg. The silicon nitride windows allow multimodal analysis of the same quantified sample, including PIXE probing of elemental abundances, molecular identification by mass spectrometry, and isotopic quantitation of interactions. The method was used with accelerator mass spectrometry to quantify specific activities of microgram quantities of14C-labeled proteins.
