Analysis of Reaction Products of Food Contaminants and Ingredients: Bisphenol A Diglycidyl Ether (BADGE) in Canned Foods
Analysis of Reaction Products of Food Contaminants and Ingredients: Bisphenol A Diglycidyl Ether (BADGE) in Canned Foods
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DOI：
10.1021/jf904160a
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发表时间：
2010-04-28
影响因子：
6.1
通讯作者：
Castle, Laurence
中科院分区：
文献类型：
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作者：
Coulier, Leon;Bradley, Emma L.;Castle, Laurence
双酚A二缩水甘油醚(BAGE)是一种环氧化物，用于制造食品接触应用的罐头涂料。从罐头涂层迁移到食品中后，徽章级别会腐烂，新的反应产物通过与食品成分的反应而形成。食品，即金枪鱼、苹果泥和啤酒，在加工和储存前添加了BAGE，证明了BAGE的显著腐烂。生命科学启发的分析方法被成功地应用于研究BAGE与食品成分的反应，例如氨基酸和糖。通过选择性地检测BAGE与低分子食品成分的反应产物，成功地将BAGE的稳定同位素与LC分析相结合，并结合荧光检测(FLD)和高分辨率质谱仪(MS)检测，改善了BAGE的质量平衡。此外，蛋白质组学方法表明，BAGE还与多肽(来自模型系统中的蛋白质消化)和食物中的蛋白质发生反应。氨基酸、多肽和蛋白质的主要反应中心是半胱氨酸。
Bisphenol A diglycidyl ether (BADGE) is an epoxide that is used as a starting substance in the manufacture of can coatings for food-contact applications. Following migration from the can coating into food, BADGE levels decay and new reaction products are formed by reaction with food ingredients. The significant decay of BADGE was demonstrated by liquid chromatographic (LC) analysis of foodstuffs, that is, tuna, apple puree, and beer, spiked with BADGE before processing and storage. Life-science inspired analytical approaches were successfully applied to study the reactions of BADGE with food ingredients, for example, amino acids and sugars. An improved mass balance of BADGE was achieved by selective detection of reaction products of BADGE with low molecular weight food components, using a successful combination of stable isotopes of BADGE and analysis by LC coupled to fluorescence detection (FLD) and high-resolution mass spectrometric (MS) detection. Furthermore, proteomics approaches showed that BADGE also reacts with peptides (from protein digests in model systems) and with proteins in foods. The predominant reaction center for amino acids, peptides, and proteins was cysteine.