Immunohistochemical localization of protease-activated receptors in cerebral and testicular arterioles of rats: their dependence on arteriole size and organ-specificity.
Immunohistochemical localization of protease-activated receptors in cerebral and testicular arterioles of rats: their dependence on arteriole size and organ-specificity.
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大鼠脑和睾丸小动脉中蛋白酶激活受体的免疫组织化学定位：它们对小动脉大小和器官特异性的依赖性。
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发表时间：
2008
影响因子：
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通讯作者：
A. Ogawa
中科院分区：
文献类型：
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作者：
Toshinari Misaki;Y. Satoh;T. Saino;T. Kuroda;K. Masu;Denis Afadhali Russa;A. Ogawa
蛋白酶激活受体（PARs）表达于血管内皮和平滑肌，在血管功能中起重要作用。使用细胞内钙离子浓度（[Ca 2 +]i）成像，我们最近观察到，大脑的小-但不是大-小动脉对蛋白酶有反应，而睾丸小动脉没有反应。本研究的目的是用免疫组织化学方法研究小动脉PAR定位的异质性。与[Ca 2 +]i成像结果一致，凝血酶受体和PAR 2在脑的大小动脉中均不明显。然而，大脑的小动脉、血管平滑肌和内皮细胞对凝血酶受体和PAR 2表现出明显的免疫反应性。PAR在睾丸小动脉中的免疫反应较弱。总之，小动脉对蛋白酶的大小依赖性和/或器官特异性反应是由于PAR的异质性定位。
Protease-activated receptors (PARs) expressed in the endothelia and smooth muscles of vessels may play important roles in blood vessel function. Using intracellular calcium ion concentration ([Ca2+]i) imaging, we recently observed that small - but not large - arterioles of the brain responded to proteases, while testicular arterioles showed no response. The purpose of the present study was to examine the heterogeneity of the localization of PARs in arterioles using immunohistochemistry. Consistent with the [Ca2+]i imaging results, neither the thrombin receptor nor PAR2 were evident in large arterioles of the brain. However, the small arterioles of the brain, vascular smooth muscles, and endothelia showed a distinct immunoreactivity against the thrombin receptor and PAR2. The immunoreactivity of PARs in testicular arterioles was faint. In conclusion, size-dependent and/or organ-specific responses of arterioles to proteases are due to the heterogeneous localization of PARs.