The effect of nicotine on the mechanical properties of mesenchymal stem cells.
The effect of nicotine on the mechanical properties of mesenchymal stem cells.
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尼古丁对间充质干细胞机械性能的影响。
DOI：
10.2147/chc.s24381
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发表时间：
2012-03-28
期刊：
影响因子：
--
通讯作者：
Cheung HS
中科院分区：
文献类型：
--
作者：
Ruiz JP;Pelaez D;Dias J;Ziebarth NM;Cheung HS
测定体外培养的人骨髓间充质干细胞（MSCs）细胞核和细胞质的弹性以及尼古丁对MSCs的影响。通过血清剥夺技术使MSC同步于细胞周期的G0期。然后用含尼古丁（0.1 µM、0.5 µM和1 µM）的培养基处理细胞。然后用原子力显微镜测量这些细胞的细胞核和细胞质的杨氏模量。对于非同步化和同步化的细胞，细胞核比细胞质软，尽管这种差异没有被发现是统计学上显著的。对于所有浓度，用尼古丁处理的细胞核比对照组显著更硬。仅在0.5 µM和1.0 µM浓度下，尼古丁处理细胞的细胞质比对照细胞显著更硬。这项研究的结果可能表明，尼古丁以剂量依赖性方式影响人MSC的生物物理特性，这可能使细胞对机械诱导和其他物理刺激的反应较低。
To measure the elasticity of the nucleus and cytoplasm of human mesenchymal stem cells (MSCs) as well as changes brought about by exposure to nicotine in vitro. MSCs were synchronized to the G0 stage of the cell cycle through serum deprivation techniques. The cells were then treated with medium containing nicotine (0.1 µM, 0.5 µM, and 1 µM). Atomic force microscopy was then used to measure the Young’s modulus of both the nucleus and cytoplasm of these cells. For both unsynchronized and synchronized cells, the nucleus was softer than the cytoplasm, although this difference was not found to be statistically significant. The nucleus of cells treated with nicotine was significantly stiffer than the control for all concentrations. The cytoplasm was significantly stiffer in nicotine-treated cells than in control cells for the 0.5 µM and 1.0 µM concentrations only. The results of this study could suggest that nicotine affects the biophysical properties of human MSCs in a dose-dependent manner, which may render the cells less responsive to mechanoinduction and other physical stimuli.