Effect of Artificial Media, Temperature Conditions and Storage Methods on in vitro Germination of Dragon Fruit (Hylocereus undatus Britt & Rose) Pollen
Effect of Artificial Media, Temperature Conditions and Storage Methods on in vitro Germination of Dragon Fruit (Hylocereus undatus Britt & Rose) Pollen
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人工培养基、温度条件和贮藏方法对火龙果离体萌发的影响
DOI：
10.11248/jsta1957.50.51
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
Y. Yonemoto
中科院分区：
文献类型：
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作者：
M. M. Macha;A. K. Chowdhury;T. Murata;Y. Yonemoto
白火龙果（同义：火龙果）（WD）是自交亲和的，而红火龙果（RD）品种是不亲和的。因此，RD品种需要人工授粉其他品种的花粉才能坐果。本研究的目的是利用不同糖浓度和不同温度的人工培养基，确定WD品种花粉作为传粉媒介的适宜萌发条件。并对各种花粉贮藏方法进行了评价。用蔗糖含量为30%的琼脂处理，花粉发芽率和试管伸长率最高。在浓度低于20%和高于40%时花粉萌发率降低，在无蔗糖和50%蔗糖浓度下花粉不萌发。发芽率在30、35和40•Ž时最高，高于45•Ž和低于25•Ž时发芽率下降。花粉萌发在10和55•Ž均未发生。新鲜花粉和未加硅胶保存20 h的新鲜花粉的花粉发芽率均超过30%。然而，在干燥条件下，用硅胶在5•Ž下储存花粉后，花粉没有萌发。在-20和-30•Ž条件下保存15天后，花粉萌发率和管伸长率下降。花粉在丙酮和乙酸乙酯中贮存后不发芽。在20% ~ 60% w/v的蔗糖溶液中，花粉存活时间为3 d，而在不含蔗糖和5%、10%蔗糖溶液中，花粉不萌发。结果表明，在30、35和40•Ž条件下，30%浓度的蔗糖有利于火龙果花粉的萌发和管伸长，而防止花粉暴露于干燥或过湿的条件是火龙果花粉贮藏的必要条件。
White fresh dragon fruit (synonym: pitaya) (WD) is self compatible, while the Red fresh dragon fruit (RD) cultivars are incompatible. Therefore, RD cultivars require artificial pollination with pollen from other cultivars to set fruit. The objective of the present study was to identify suitable conditions for pollen germination of WD cultivars as a pollinator, using artificial media at various sugar concentrations and various temperatures. Also various pollen storage methods were evaluated . The use of agar containing 30% sucrose led to the highest values for the percentage of pollen germination and tube elongation. The percentage of pollen germination was reduced at concentrations below 20% and above 40%, and the pollen did not germinate at in the absence of sucrose and at a 50% sucrose concentration. Although the highest germination percentage was observed at 30, 35 and 40•Ž, the germination percentage decreased at temperatures above 45•Ž and below 25•Ž . Pollen germination did not occur at 10 and 55•Ž. Pollen germination percentage of fresh pollen and fresh pollen stored for 20 hrs without silica gel exceeded 30%. However, pollen germination did not occur after storage under dry conditions with silica gel at 5•Ž. Pollen germination and tube elongation were reduced after storage at -20 and -30•Ž for 15 days. Pollen did not germinate after being stored in acetone and ethyl acetate. Pollen stored in a sucrose solution (from 20% to 60% w/v) remained viable for 3 days, while pollen germination did not occur after pollen was stored in the absence of sucrose or in 5 and 10% sucrose solutions. It was concluded that the use of sucrose at 30% concentration at 30, 35, and 40•Ž was suitable for pollen germination and tube elongation, while prevention of exposure of pollen to dry or too wet conditions is necessary for pollen storage of dragon fruit .