Enhancing angiogenesis alleviates hypoxia and improves engraftment of enteric cells in polycaprolactone scaffolds.
Enhancing angiogenesis alleviates hypoxia and improves engraftment of enteric cells in polycaprolactone scaffolds.
复制标题
增强血管生成可缓解缺氧并改善肠细胞在聚己内酯支架中的植入。
DOI：
10.1002/term.1484
复制
发表时间：
2012
影响因子：
3.3
通讯作者：
Dunn,JamesCY
中科院分区：
文献类型：
--
作者：
Singh,Shivani;Wu,BenjaminM;Dunn,JamesCY
我们研究了在多孔聚己内酯(PCL)支架中加速血管生成是否可以减少缺氧，从而提高移植的肠道细胞的存活率。为了加速血管生成，我们使用表面交联型肝素的PCL支架运送血管内皮生长因子(VEGF)。支架的制备和表征已经在我们之前的研究中得到了报道。从新生小鼠肠道组织分离的肠上皮细胞体外培养10 后，其收缩蛋白α-平滑肌肌动蛋白和结蛋白的表达水平较高。将培养的肠上皮细胞接种于支架内，分别接种于免疫缺陷小鼠皮下7天和14 。在第7天，含有血管内皮生长因子的肝素修饰的PCL支架显著增加了血管生成和肠道细胞的植入，同时减少了缺氧。在第14天，血管在支架的整个厚度上生长，导致缺氧环境显著减少；然而，移植的细胞密度没有进一步增加。综上所述，血管生成的增强减少了细胞的缺氧，并改善了肠道细胞的植入。版权所有©2012 John Wiley&Sons，Ltd.
We examined whether expediting angiogenesis in porous polycaprolactone (PCL) scaffolds could reduce hypoxia and consequently improve the survival of transplanted enteric cells. To accelerate angiogenesis, we delivered vascular endothelial growth factor (VEGF) using PCL scaffolds with surface crosslinked heparin. The fabrication and characterization of scaffolds has been reported in our previous study. Enteric cells, isolated from intestinal tissue of neonatal mice and expandedin vitrofor 10 days, exhibited high expression levels for contractile proteinα‐smooth muscle actin and desmin. The cultured enteric cells were seeded in scaffolds and were implanted subcutaneously in immunodeficient mice for 7 and 14 days. At day 7, the heparin‐modified PCL scaffolds with VEGF exhibited significantly increased angiogenesis and engraftment of enteric cells, with a simultaneous reduction in hypoxia. At day 14, the blood vessels grew across the entire thickness of the scaffold and resulted in a significantly diminished hypoxic environment; however, the transplanted cell density did not increase further. In conclusion, the enhancement of angiogenesis reduced cellular hypoxia and improved the engraftment of enteric cells. Copyright © 2012 John Wiley & Sons, Ltd.