UV Treatment Improves the Biocompatibility and Antibacterial Properties of Crystallized Nanostructured Titanium Surface
UV Treatment Improves the Biocompatibility and Antibacterial Properties of Crystallized Nanostructured Titanium Surface
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DOI：
10.3390/ijms20235991
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发表时间：
2019-12-01
影响因子：
5.6
通讯作者：
Okazaki, Joji
中科院分区：
文献类型：
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作者：
Hatoko, Mai;Komasa, Satoshi;Okazaki, Joji
本研究描述了一种由具有结晶纳米结构的纯钛（Ti）金属组成的新材料的生产，并研究了热处理和紫外线（UV）照射是否改善了其生物相容性和抗菌性能。我们比较了通过暗碱性处理和在600摄氏度下加热形成的UV照射和未照射的Ti纳米片（TNS）与未处理的纯Ti纳米结构（阳性对照）的性能。使用人脐静脉内皮细胞和大鼠骨髓细胞进行体外和体内实验，以评估生物相容性和对细胞行为的影响。用X射线光电子能谱（XPS）对材料表面进行了表征。使用金黄色葡萄球菌（一种常见的致病菌）评价辐照材料的抗菌性能。紫外线照射的TNS表现出较高的血管生成能力，并促进细胞粘附和分化相对于控制。此外，通过XPS的表面分析揭示了UV处理的材料的较低的C峰，表明材料表面上的污垢量减少。紫外线照射降低了S.金黄色葡萄球菌在材料表面上通过刺激活性氧产生。经紫外线照射后，TNS的生物相容性和抗菌性能得到改善。因此，TNS可以作为一种有用的材料用于制造牙科植入物。
This study describes the production of a new material composed of pure titanium (Ti) metal with a crystallized nanostructure and investigated whether heat treatment and ultraviolet (UV) irradiation improved its biocompatibility and antibacterial properties. We compared the performance of UV-irradiated and non-irradiated Ti nanosheets (TNS) formed by dark alkaline treatment and heating at 600 degrees C with that of untreated pure Ti nanostructure (positive control). In vitro and in vivo experiments to assess biocompatibility and effects on cell behavior were performed using human umbilical vein endothelial cells and rat bone marrow cells. The material surface was characterized by X-ray photoelectron spectroscopy (XPS). The antibacterial properties of the irradiated material were evaluated using Staphylococcus aureus, a common pathogenic bacterium. The UV-irradiated TNS exhibited high angiogenic capacity and promoted cell adherence and differentiation relative to the control. Further, surface analysis via XPS revealed a lower C peak for the UV-treated material, indicating a reduced amount of dirt on the material surface. Moreover, UV irradiation decreased the viability of S. aureus on the material surface by stimulating reactive oxygen species production. The biocompatibility and antibacterial properties of the TNS were improved by UV irradiation. Thus, TNS may serve as a useful material for fabrication of dental implants.