A novel nitrogen-dependent gene associates with the lesion mimic trait in wheat
A novel nitrogen-dependent gene associates with the lesion mimic trait in wheat
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DOI：
10.1007/s00122-016-2758-3
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发表时间：
2016-11-01
影响因子：
5.4
通讯作者：
Li, Tao
中科院分区：
文献类型：
--
作者：
Li, Lei;Shi, Xuan;Li, Tao
利用批量分离分析（BSA）结合RNA-seq和DNA标记，在小麦2BS上发现了一个潜在的氮依赖型病斑模拟基因Ndhrl 1。在小麦品系P7001中，LM在0 g氮（N）处理下出现在叶片上，但在足够的N供应下消失，表明LM是N响应的和N剂量依赖的。使用BSA策略结合RNA-seq和DNA标记，我们鉴定了N依赖性LM基因（Ndhrl 1），并使用由P7001 x P216杂交开发的F-5重组近交群体将其定位到染色体2B的短臂。推定基因被界定为8.1 cM的区间，两侧是CAPS/dCAPS标记7 hrC 9和7 hr 2dc 14，并与dCAPS标记7 hrdc 2共分离。根据其染色体定位，该基因很可能是小麦LM的一个新基因。RNA-seq数据的进一步分析表明，植物-病原体互作、氮代谢、玉米素生物合成和植物激素信号转导途径在LM和非LM系之间显著差异表达。
Using bulk segregant analysis (BSA) coupling with RNA-seq and DNA markers identified a potentially novel nitrogen-dependent lesion mimic gene Ndhrl1 on 2BS in wheat.Lesion mimic (LM) refers to hypersensitive reaction-like (HRL) traits that appear on leaf tissue in the absence of plant pathogens. In a wheat line P7001, LM showed up on the leaves under the 0 g nitrogen (N) treatment, but disappeared when sufficient N was supplied, suggesting that LM is N-responsive and N dosage dependent. Using BSA strategy together with RNA-seq and DNA markers, we identified an N-dependent LM gene (Ndhrl1) and mapped it to the short arm of chromosome 2B using an F-5 recombinant inbred population developed from the cross of P7001 x P216. The putative gene was delimited into an interval of 8.1 cM flanked by the CAPS/dCAPS markers 7hrC9 and 7hr2dc14, and co-segregated with the dCAPS marker 7hrdc2. This gene is most likely a novel gene for LM in wheat based on its chromosomal location. Further analysis of RNA-seq data showed that plant-pathogen interaction, nitrogen metabolism, zeatin biosynthesis and plant hormone signal transduction pathways were significantly differentially expressed between LM and non-LM lines.